Sequences from confirmation amplicon


Confirmation sequences for line 266D04 and hit At5g45600 are shown.
Line ID 266D04
Locus At5g45600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18489655
Primers used for PCR AC15 - 8474, show primer details
   
Sequence Name 68-K028102-022-266-D04C-8409
GenBank Accession LN511923 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K028102-022-266-D04C-8409
TTTACAATTGAATATATCCTGTTTTAACGGTCTGATTCTGTCCCTCTAGCAAACTTATTT
GTCGCCTCAGTTTCGCAATATGAGCTTGAACCTATCCAACAAAGAACCAATCCAAATTCA
TCAAAACATAGACCAACAAAAATAGTCTAAACTGAGTATCAAAAATTTAGAAAACACAGT
TAAATGTTTTAAAGTTACCTGTTGACGAGCAGCTGCAAGCTGTAATAACTCATCTGCTTC
AGAGAAACTCATGAACCATTGTCCCAAACTATGATCTTTCGTATCACCTCTCTTCTTTTT
CCCGGTATCTTCGACTTGCACTAGTCATAAGATTTGTTGTGTGAGACGACATTTTAAGGC
AATGATCAACTTAAACTAAGGAATGAAAAAAGCTTTAGATAAGGAGATCATACTAGGAGC
AGGCAAGTTGTAACCAGAGGGTAATCTTGGAAAGGTCAAAGCCGGATGATTCTGAACCCT
AGCTAAAAAACTTTCTGAAGGATCAGGAAAGACAATCTCGTCATAAGATTCCACAACCAC
AGGCTTCTTCATTGTCAAAGGACCTGATTCATCTTCAGGGTATAGCTTTAAATGATGATA
TCTGTTG
   
Sequence Name 68-K028102-022-266-D04C-AC15
GenBank Accession LN511924 [GenBank]
Sequencing primer gene specific primer, AC15: 5'-TGTTTTGGGTGGATACAACAGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K028102-022-266-D04C-AC15
CATGAAGAAGCCTGTGGTTGTGGAATCTTATGACGAGATTGTCTTTCCTGATCCTTCAGA
AAGTTTTTTAGCTAGGGTTCAGAATCATCCGGCTTTGACCTTTCCAAGATTACCCTCTGG
TTACAACTTGCCTGCTCCTAGTATGATCTCCTTATCTAAAGCTTTTTTCATTCCTTAGTT
TAAGTTGATCATTGCCTTAAAATGTCGTCTCACACAACAAATCTTATGACTAGTGCAAGT
CGAAGATACCGGGAAAAAGAAGAGAGGTGATACGAAAGATCATAGTTTGGGACAATGGTT
CATGAGTTTCTCTGAAGCAGATGAGTTATTACAGCTTGCAGCTGCTCGTCAACAGGTAAC
TTTAAAACATTTAACTGTGTTTTCTAAATTTTTGATACTCAGTTTAGACTATTTTTGTTG
GTCTATGTTTTGATGAATTTGGATTGGTTCTTTGTTGGATAGGTTCAAGCTCATATTGCG
AAACTGAGGCGACAAATAAGTTTGCTAGAGGGACAGAATCAGACCGTTAAAACAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACG
TGTCT

Last Updated on 06.12.2018 16:11