Sequences from confirmation amplicon


Confirmation sequences for line 266H09 and hit At4g23570 are shown.
Line ID 266H09
Locus At4g23570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12301309
Primers used for PCR AY26 - 8474, show primer details
   
Sequence Name 49-K018912-022-266-H09C-AY26
GenBank Accession LN506679 [GenBank]
Sequencing primer gene specific primer, AY26: 5'-TGCCTGTATGAAGCTTGAAGAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K018912-022-266-H09C-AY26
CTAAAACAGCTCTTGAAAAGGGTGCCTCTATCACGCCGAGTGAATCCAAATTTAAGAAGT
TGATAGATGAATGCAATTTTCTAATCACAGGTATGTTTTTTGTGTTTTTTTCTTGTTTTC
TGGTTGATTATGTTTACATGCCTAGAGTGATTGTATTGTTTGCCATGGTGCAAAATATTT
TGTTATGTAATTCACCTAAGGTTTTGATCTATGGAACTTGTAAATATCCTTTTTCCAGAA
GAAGAGAAAGATTTGGTTCAACCGGAAAAAGAAATCCGGGAAATATATACAATTCAGTGG
AAAAGATTTAAGAATCAACAATCTACATGTAGGTTTATACCATGTGGATTTTGTCGTTTT
ACATGAAGCCATTTGATCCATGTATTGTTGAATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
   
Sequence Name 53-K018912-022-266-H09C-8409
GenBank Accession LN507998 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K018912-022-266-H09C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATTCAACAATACATGGAT
CAAATGGCTTCATGTAAAACGACAAAATCCACATGGTATAAACCTACATGTAGATTGTTG
ATTCTTAAATCTTTTCCACTGAATTGTATATATTTCCCGGATTTCTTTTTCCGGTTGAAC
CAAATCTTTCTCTTCTTCTGGAAAAAGGATATTTACAAGTTCCATAGATCAAAACCTTAG
GTGAATTACATAACAAAATATTTTGCACCATGGCAAACAATACAATCACTCTAGGCATGT
AAACATAATCAACCAGAAAACAAGAAAAAAACACAAAAAACATACCTGTGATTAGAAAAT
TGCATTCATCTATCAACTTCTTAAATTTGGATTCACTCGGCGTGATAGAGGCACCCTTTT
CAAGAGCTGTTTTAGCAGTCCGATACTCTTCAAGCTTCAAACAGG

Last Updated on 06.12.2018 16:11