Sequences from confirmation amplicon


Confirmation sequences for line 267D01 and hit At1g32340 are shown.
Line ID 267D01
Locus At1g32340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11666018
Primers used for PCR TN04 - 8474, show primer details
   
Sequence Name 45-K105521-0022-267-D01C-8409
GenBank Accession LN505531 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K105521-0022-267-D01C-8409
ATTTCTCNTTACATTTTGCTGCTGGACATGTAGATTGCAGTGGAAGAAATCACGACCGCC
GCTACATCCGCCGTGACGCTGGGATTGTGAATCCAGTCAACGATCATCCAAAATCAGAGC
ATCAGAAAGAAGACACGACTTCGACGGATTTATCATCTGCAGCAGCTTTTCCGTCTACTT
CTCAGCCAATCCAAAACCTCGATCATTCTACAAAACCTTCAAAGTCTCACCGGAATCGAC
GCAGCCGTGGATCAAATTCAAAACCTCGTCCCGTGGAGAAACCGGAGGTGAATTTTGCTG
AATCCGATGTTGTTGATTGCTTGGCTGATGAATTGAGTAGGTTAAAGGTGAAACAGAATT
CGCATTCGGTGAATATGGAGGAGAAGTTTCATCACAGTTC
   
Sequence Name 46-K105521-0022-267-D01C-OriL
GenBank Accession LN505865 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K105521-0022-267-D01C-OriL
GCCATTTCTCATTACATTTTGCTGCTGGACATGTAGATTGCAGTGGAAGAAATCACGACC
GCCGCTACATCCGCCGTGACGCTGGGATTGTGAATCCAGTCAACGATCATCCAAAATCAG
AGCATCAGAAAGAAGACACGACTTCGACGGATTTATCATCTGCAGCAGCTTTTCCGTCTA
CTTCTCAGCCAATCCAAAACCTCGATCATTCTACAAAACCTTCAAAGTCTCACCGGAATC
GACGCAGCCGTGGATCAAATTCAAAACCTCGTCCCGTGGAGAAACCGGAGGTGAATTTTG
CTGAATCCGATGTTGTTGATTGCTTGGCTGATGAATTGAGTAGGTTAAAGGTGAAACAGA
ATTCGCATTCGGTGAATATGGAGGAGAAGTTTCATCACA

Last Updated on 06.12.2018 16:11