Sequences from confirmation amplicon


Confirmation sequences for line 267D10 and hit At5g44290 are shown.
Line ID 267D10
Locus At5g44290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17840687
Primers used for PCR NH37 - 8474, show primer details
   
Sequence Name 27-K036523-022-267-D10C-8409
GenBank Accession LN498293 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K036523-022-267-D10C-8409
GAGCCATTTAAATTGCTGGAATGGTTCTCTGTTTTTGTCCTCTGATAAAAGACTCCCAGC
AAAATCATTCAGATACGTGATTTAGTTTCTGCAGTGTTTTGCTTTCTGTCTGGTGAAATC
TTGGAGCTGCCGGTCGTGTTCTTCCAGCATCTGATCCACTTTTCTTGGCTGAGCCAGTAA
TGGACCGGACATGTACATCTTGTTATCCCCTGAATCATACTATATCTCCAAAGAGAGAGA
GAGAGATAAAGACAATGTAACTGAGAAATGAGGAAAAAAATAGTCTCTTGAGATGACTTA
CCCAAGATGGGTCATTTGGGATTTTGGTTTGTCTTTTACTATTATCCATAGTGTCAGCTC
TGTTGAATGTTCTCAATTCCTTCCTTTGCTGATCCATCATTTGTTGATCTGACCCAACAT
AACCGCCTCCTTGTCCTGCTGAGTTAGCCCTATATGAAGGTATTCTAGGGGCTGCATTGT
CCTTCACGTATGTTAACCGACTAAGATTCCGGTTTTTCATCATCGGACCTGAGTGAGAGA
CTCTTCCGCTTGTTGGATTGTTGCAGGTCATGTCCTGCATTTGT
   
Sequence Name 51-K036523-022-267-D10C-NH37
GenBank Accession LN507423 [GenBank]
Sequencing primer gene specific primer, NH37: 5'-GGTAATTCACAAAGACAAATGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K036523-022-267-D10C-NH37
CACAGCGCGAGAGTCTCTCACTCAGGTCCGATGATGAAAAACCGGAATCTTAGTCGGTTA
ACATACGTGAAGGACAATGCAGCCCCTAGAATACCTTCATATAGGGCTAACTCAGCAGGA
CAAGGAGGCGGTTATGTTGGGTCAGATCAACAAATGATGGATCAGCAAAGGAAGGAATTG
AGAACATTCAACAGAGCTGACACTATGGATAATAGTAAAAGACAAACCAAAATCCCAAAT
GACCCATCTTGGGTAAGTCATCTCAAGAGACTATTTTTTTCCTCATTTCTCAGTTACATT
GTCTTTATCTCTCTCTCTCTCTTTGGAGATATAGTATGATTCAGGGGATAACAAGATGTA
CATGTCCGGTCCATTACTGGCTCAGCCAAGAAAAGTGGATCAGATGCTGGAAGAACACGA
CCGGCAGCTCCAAGATTTCACCAGACAGAAAGCAAAACACTGCAGAAACTAAATCACGTA
TCTGAATGATTTTGCTGGGAGTCTTTTATCAGAGGACAAAAACAGAGAACCATTCCAGCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
ACATTCA

Last Updated on 06.12.2018 16:11