Sequences from confirmation amplicon


Confirmation sequences for line 268A11 and hit At3g02000 are shown.
Line ID 268A11
Locus At3g02000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:332631
Primers used for PCR SZ09 - 8474, show primer details
   
Sequence Name 31-K023623-022-268-A11C-8409
GenBank Accession LN499990 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K023623-022-268-A11C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGCCAAATGTAGATTCTCT
CCCGGTCGTCTTCATCGGCGGTAAACTGGTTGGAGCTATGGACAGAGTCATGGCTTCTCA
CATAAACGGCTCTCTCGTTCCTCTTCTCAAAGACGCCGGCGCTCTCTGGCTCTGATCCCT
TCCTCTGCTTTCTTTTTTCTTTTCTATTTGAAGTTTTCTTGTAAGAGAATGTGGTGGAGG
AAGATTAGGAAACTAGTCAATGGCTGTAATGACAGGTTTTAGATTATAGTTTGTAATTAG
AGAGAGAGTTGTTTTAAGCTCACCTTTCTCTGTCTTCCTCTTCTTCATCTCTTATTGATC
TTTCGAATGCTCTCATTAAATCATAATAGTAAACACTTTGCATCTTTATTAATGTCCCCT
GGATCCATTAATCAACGGTATTAACTGACGCCGTA
   
Sequence Name 32-K023623-022-268-A11C-SZ09
GenBank Accession LN500416 [GenBank]
Sequencing primer gene specific primer, SZ09: 5'-TTTGAATAGATACGGCGTCAGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K023623-022-268-A11C-SZ09
ATACCGTTGATTAATGGATCCAGGGGACATTAATAAAGATGCAAAGTGTTTACTATTATG
ATTTAATGAGAGCATTCGAAAGATCAATAAGAGATGAAGAAGAGGAAGACAGAGAAAGGT
GAGCTTAAAACAACTCTCTCTCTAATTACAAACTATAATCTAAAACCTGTCATTACAGCC
ATTGACTAGTTTCCTAATCTTCCTCCACCACATTCTCTTACAAGAAAACTTCAAATAGAA
AAGAAAAAAGAAAGCAGAGGAAGGGATCAGAGCCAGAGAGCGCCGGCGTCTTTGAGAAGA
GGAACGAGAGAGCCGTTTATGTGAGAAGCCATGACTCTGTCCATAGCTCCAACCAGTTTA
CCGCCGATGAAGACGACCGGGAGAGAATCTACATTTGGCCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11