Sequences from confirmation amplicon


Confirmation sequences for line 268B10 and hit At3g46440 are shown.
Line ID 268B10
Locus At3g46440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17089408
Primers used for PCR MP11 - 8474, show primer details
   
Sequence Name 50-K017191-022-268-B10C-MP11
GenBank Accession LN507015 [GenBank]
Sequencing primer gene specific primer, MP11: 5'-TATAGTTGAATGACTTCGCCATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K017191-022-268-B10C-MP11
AGTCACCGTAGCTGCGTAAGCTATGGCAAGAAAGTAAATTTTATCTTCCTTAAATATAAA
AAGCATTCGTTCATAATCTTTATTTTTCTCTAGGAATAATTTCGACTGTGGAAAGTAGAA
ACATATTATATGAAACCAACCGCTTGCACCATGGAATATGAAAAGTAGAACTTGGTTATG
TAGTGCATTCACGTTTGCGTGTGATCGGTGATGGAGTTTTAGTTCTTGTGGACTCCGAGC
CTTAGCCTGAAGTCTTCTTCCATAAGTGGAAGGCCTTCACGAAGCTTCACCTTTGGTTCC
CATCCCAACACTTCTTTAGCCTTTGCGATGTCTGGCTTCCTCTGTCTTGGATCCATCCGG
CGCGCTCTCA
   
Sequence Name 54-K017191-022-268-B10C-8409
GenBank Accession LN508323 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K017191-022-268-B10C-8409
ATATAGATTTTCGGACATGAAGCCATTTACAATTGAATATATGATATGATATGATATGAT
ATGAGTACAGTACTTAGAAATTCTTGGGTCCGCTAATGAAATGTTGGTTTACTTGCAGCT
GATTAACCCGAGCATAGAGATAAAGATGGTTGACCAACACACCGGCAGATCCAAGACAGA
GGAAGCCAGACATCACCAAGGCTAAAGAAGCGCCGGCATGGGAACCAAAGGTGAAGCTTC
CTGAAGGCCCTCC

Last Updated on 06.12.2018 16:11