Sequences from confirmation amplicon


Confirmation sequences for line 269C05 and hit At5g01850 are shown.
Line ID 269C05
Locus At5g01850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:333634
Primers used for PCR KR48 - 8474, show primer details
   
Sequence Name 61-K100821-0022-269-C05C-8409
GenBank Accession LN510455 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K100821-0022-269-C05C-8409
GAGCCATTTACAATTGGATGACACGAACACAGACATGCATATATGGCTTTTTTATAATGT
CAAATAGTGGTATGATTTACAAGAATGACAGAGCGGGCAAAAACCTCAGGAGCCATCCAA
CGGTAAGTCCCAGTCTCAGCAGTCATCATCTCAGTCACGGATTCTTCCCTAGCAAGCCCG
AAATCAGCAAGCTTGACGGATTTGTGATTCTCCGTGAGCAATAAGTTGTCTGCGACATTG
GTTAACTTATGAGTCCAGTAGTTAAAACATAAAAGGTGTGCTAGGAAGGAAGCAGGGATA
GAAGAGTAGTACCAGGTTTGAGGTCTCTGTGAATGATACCATTGGCGTGTAAGCAGTGCA
AGGCACGGGCGATGTCAAGGGCAAAGGAGAGAGCAAGAGGGAGATGGAGCAACTGAGGAC
GGATGCTGGTGAGATATTTACGGAGAGACATCCCTGGGAGAAGCTCTGTTACTATTACCA
TTAAAGGATCTTTGCACGCTCCAATAAACTGCCAAATGGATATTGTGTATTCAAGAAGAA
GTATCGAGAGCAAAGAAAGAGAAGAAAGAAGGAGAGANCCCTTGACA
   
Sequence Name 61-K100833-0022-269-C05C-KR48
GenBank Accession LN510457 [GenBank]
Sequencing primer gene specific primer, KR48: 5'-TGTCAAGGTCTCTCTCCTTCTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K100833-0022-269-C05C-KR48
TCTTCTTGATACACAATATCCATTTGGCAGTTTATTGGAGCGTGCAAAGATCCTTTAATG
GTAATAGTAACAGAGCTTCTCCCAGGGATGTCTCTCCGTAAATATCTCACCAGCATCCGT
CCTCAGTTGCTCCATCTCCCTCTTGCTCTCTCCTTTGCCCTTGACATCGCCCGTGCCTTG
CACTGCTTACACGCCAATGGTATCATTCACAGAGACCTCAAACCTGGTACTACTCTTCTA
TCCCTGCTTCCTTCCTAGCACACCTTTTATGTTTTAACTACTGGACTCATAAGTTAACCA
ATGTCGCAGACAACTTATTGCTCACGGAGAATCACAAATCCGTCAAGCTTGCTGATTTCG
GGCTTGCTAGGGAAGAATCCGTGACTGAGATGATGACTGCTGAGACTGGGACTTACCGTT
GGATGGCTCCTGAGGTTTTTGCCCGCTCTGTCATTCTTGTAAATCATACCACTATTTGAC
ATTATAAAAAAGCCATATATGCATGTCTTGTGTTCGTGTCATCCAATTGTAAATGGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT

Last Updated on 06.12.2018 16:11