Sequences from confirmation amplicon


Confirmation sequences for line 269C11 and hit At4g09150 are shown.
Line ID 269C11
Locus At4g09150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5832150
Primers used for PCR 07wu - 8474, show primer details
   
Sequence Name 85-K118926-0022-269-C11C-07wu
GenBank Accession LN514594 [GenBank]
Sequencing primer gene specific primer, 07wu: 5'-CAGGTGAATTTTACCAGTTGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K118926-0022-269-C11C-07wu
TATCCCAAAAGATAGCTAAAATTGATAGATGGACACCAAACCTGGAAATGGAGATGAAGG
GGTATTGACTTTTGAAACAGAGGAGACATTGCCACCAGTTCGCATGGTGGTCGAAGGGAG
GCCTGATGATCCAGAGTGGTTATTTATGACAGCAGAAAGAGCATCTTTGTGTTCTTTCCA
CTCCCTCTCTGCTTCTCCTCGAACACTTAAAAGCCAACGTTTTGTCAATGGTAGCGAGGA
AGACGCTTGGTCTGGGGAACCATGCCGACTGGAAAATGACTTCTTCAGATATTCCAGACC
AGCAGGTCCCTTAAGAAGTGGCTCCAAGAGTTTCAAACGAGATTTGCTGATTTCCTTCTT
TAGAATCTGCAAGTAAAAGGACGTATGACGTTAAAAGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATTA
   
Sequence Name 61-K118925-0022-269-C11C-8409
GenBank Accession LN510633 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K118925-0022-269-C11C-8409
GCCTTTTACGTCATACGTCCTTTTACTTGCAGATTCTAAAGAAGGAAATCAGCAAATCTC
GTTTGAAACTCTTGGAGCCACTTCTTAAGGGACCTGCTGGTCTGGAATATCTGAAGAAGT
CATTTTCCAGTCGGCATGGTTCCCCAGACCAAGCGTCTTCCTCGCTACCATTGACAAAAC
GTTGGCTTTTAAGTGTTCGAGGAGAAGCAGAGAGGGAGTGGAAAGAACACAAAGATGCTC
TTTCTGCTGTCATAAATAACCACTCTGGATCATCAGGCCTCCCTTCGACCACCATGCGAA
CTGGTGGCAATGTCTCCTCTGTTTCAAAAGTCAATACCCCTTCATCTCCATTTCCAGGTT
TGGTGTCCATCTATCAATTTTAGCTATCTTTTTGGGATTATTTTTGTAAGAACTTAGTTT
AGCGAACACAAACCAACTGGTAAAATTCACCT

Last Updated on 06.12.2018 16:11