Sequences from confirmation amplicon


Confirmation sequences for line 269E12 and hit At3g54670 are shown.
Line ID 269E12
Locus At3g54670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20238303
Primers used for PCR ND16 - 8474, show primer details
   
Sequence Name 07-K032004-022-269-E12C-8409
GenBank Accession LN489155 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K032004-022-269-E12C-8409
AATGTATTTCGCCTGCTCAGAAGGGGTTTATACTATGGATAGGGAAGATGGAAAATAATG
TGTTCTTGAAAAATGAATTACCAATATCTCTCAAGCCATAGTTCAAAAGATGAAGCAACC
GCAAGAAGAATCTCTAAGGAGGTTTTAAAAAAACTTGCAGCAATGTGTCGCATTTAAAGG
ATATCATAACATCGCAAGTAAACCATCCATTGCTAGACAACCTAGTGAGTAGAGGACATA
AACTAACCTGGCATTTACGTGCTTCTCTTGCAGTGCACGTAACTCCGTTTTCAGACTAGT
TGTTTCATTCTTATATTTCGACGAAGATGTTTCAATCTCTCCTTGCCTGTCTTTAAATCG
CTTAATTTGTTCATCAAGATCATTCTTCCTATTTATTAGTTGCTGGTAATTTCCTTCCAG
   
Sequence Name 08-K032004-022-269-E12C-ND16
GenBank Accession LN489621 [GenBank]
Sequencing primer gene specific primer, ND16: 5'-TCTGGAAGAAAATTACCAGCAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K032004-022-269-E12C-ND16
TTGATGACAATTAAGCGATTTAAGACAGGCAAGGAGAGATTGAAACATCTTCGTCGAAAT
ATAAGAATGAAACAACTAGTCTGAAAACGGAGTTACGTGCACTGCAAGAGAAGCACGTAA
ATGCCAGGTTAGTTTATGTCCTCTACTCACTAGGTTGTCTAGCAATGGATGGTTTACTTG
CGATGTTATGATATCCTTTAAATGCGACACATTGCTGCAAGTTTTTTTAAAACCTCCTTA
GAGATTCTTCTTGCGGTTGCTTCATCTTTTGAACTATGGCTTGAGAGATATTGGTAATTC
ATTTTTCAAGAACACATTATTTTCCATCTTCCCTATCCATAGTATAAACCCCTTCTTGAG
CAGGCGAAATACATTTTGTATCCCCTGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACATTCA
Primers used for PCR FP82 - 8474, show primer details
   
Sequence Name 41-K035337-022-269-E12C-8409
GenBank Accession LN503990 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K035337-022-269-E12C-8409
GAGCCATGTATACTCATCTGCTGCTGTACTGAATTCCTAGCTAAACCAAAATGAAGCTTG
TAGGCTCGTAGAGAAACCTTCAAAGTCCCTTGAGGTGCACCTCACCAGGGAGAACTTTAT
ATTTGTTCTTGATAGTTGCTAACCTGCTTATATGATGAACAGGGAGGCCTCTGCGAAGTT
GAAGACTAGAATCGCGGAATTAGAAGACCAGTTAAGTGATTTAACGGCTGAAAGGTATGA
AAATGAGAGAGATAGTAGGCTAACACAGGCTGTTGAGTCTCTCAAGCGTCTATTTCAAGG
AGTTCATGGTCGGATGACTGATCTTTGTCGGCCAAATCGGAAGAAGTATAACCTTGC
   
Sequence Name 41-K035337-022-269-E12C-FP82
GenBank Accession LN503991 [GenBank]
Sequencing primer gene specific primer, FP82: 5'-GACAGCAAGGTTATACTTCTTCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K035337-022-269-E12C-FP82
TCCGACATGAACTCCTTGAATAGACGCTTGAGAGACTCAACAGCCTGTGTTAGCCTACTA
TCTCTCTCATTTTCATACCTTTCAGCCGTTAAATCACTTAACTGGTCTTCTAATTCCGCG
ATTCTAGTCTTCAACTTCGCAGAGGCCTCCCTGTTCATCATATAAGCAGGTTAGCAACTA
TCAAGAACAAATATAAAGTTCTCCCTGGTGAGGTGCACCTCAAGGGACTTTGAAGGTTTC
TCTACGAGCCTACAAGCTTCATTTTGGTTTAGCTAGGAATTCAGTACAGCAGCAGATGAG
TATACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
ATTCACGTCCAAATGGG

Last Updated on 06.12.2018 16:11