Sequences from confirmation amplicon


Confirmation sequences for line 269F07 and hit At4g30350 are shown.
Line ID 269F07
Locus At4g30350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14848707
Primers used for PCR 14uj - 8474, show primer details
   
Sequence Name 54-K118051-0022-269-F07C-14uj
GenBank Accession LN508618 [GenBank]
Sequencing primer gene specific primer, 14uj: 5'-TTACACGTGGCAGCTACTCTCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K118051-0022-269-F07C-14uj
CAATCTCATCCAACTCTTCTCACCCACTTCAGTGTCGAGCTCTCGAGCTCTGTTTCAGCG
TCGCTCTCGAACGTCTCCCTACAACTAGTACAACAACAACCACTACTTCGTCGTCTTCTT
CTTCTTCTCCGTCGCAAACGCAAGAACCGCTACTTTCAAACGCTTTGACCGCGGCTTTAA
AACGTGCTCAAGCTCATCAACGACGTGGATGTCCTGAGCAACAGCAACAACCGCTTTTGG
CTGTGAAAGTTGAGCTTGAACAGCTTATTATATCGATTCTTGATGACCCGAGTGTGAGTC
GGGTCATGCGTGAAGCTAGCTTCTCTAGCCCCGCCGTGAAGTCAGCGATCGAACAGTCGT
TGATTGGTAACTCTGTTTCTAATTCGAGACAAACCGGCTCACCCGGAATTATTAACCCGT
CTGCAATTGGGTTCGGTTATCGTTCCGTACCGGCACCGGTTAACCGGAATCTTTATCTTA
ATCCCCGGTTACAACAACCCGGCGTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 18-K118050-0022-269-F07C-8409
GenBank Accession LN494499 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118050-0022-269-F07C-8409
TCATTGACGCCGGGTTGTTGTAACCGGGGATTAAGATAAAGATTCCGGTTAACCGGTGCC
GGTACGGAACGATAACCGAACCCAATTGCAGACGGGTTAATAATTCCGGGTGAGCCGGTT
TGTCTCGAATTAGAAACAGAGTTACCAATCAACGACTGTTCGATCGCTGACTTCACGGCG
GGGCTAGAGAAGCTAGCTTCACGCATGACCCGACTCACACTCGGGTCATCAAGAATCGAT
ATAATAAGCTGTTCAAGCTCAACTTTCACAGCCAAAAGCGGTTGTTGCTGTTGCTCAGGA
CATCCACGTCGTTGATGAGCTTGAGCACGTTTTAAAGCCGCGGTCAAAGCGTTTGAAAGT
AGCGGTTCTTGCGTTTGCGACGGAGAAGAAGAAGAAGACGACGAAGTAGTGGTTGTTGTT
GTACTAGTTGTAGGGAGACGTTCGAGAGCGACGCTGAAACAGAGCTCGAGAGCTCGACAC
TGAAGTGGGTGAGAAGAGTTTGGATGAGATTTGATACAAGCTTGTCGGAGATAACCAGAG
GAAGAAGATAAGAGAGT

Last Updated on 06.12.2018 16:11