Sequences from confirmation amplicon


Confirmation sequences for line 270D06 and hit At3g11850 are shown.
Line ID 270D06
Locus At3g11850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3739599
Primers used for PCR 18gp - 8474, show primer details
   
Sequence Name 24-K112248-0022-270-D06C-18gp
GenBank Accession LN497055 [GenBank]
Sequencing primer gene specific primer, 18gp: 5'-AATACATTTTCTCTGCAACCCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K112248-0022-270-D06C-18gp
AGTGAGAAGGCCGACAATGTTACCTCGCCAATCCCACATCAGTATTTGACATTATGTGCT
TCGAATCTAGAGTCAGGAGAGTGATTTTGTTGGCTTGAAAGGATTGGATCTGTTTCACAC
TTGCGTGCTCCTAAGACATCGCCATTTCCTTGACCGAGGGACTTTTTCTAGAAGCATTTG
CAAGCCGATGTTGTTTCCTGACATCCCATACATGTATCTGCGTATTTGCATTGGAACAGA
GACCAGCATAAGTTTCATAAGCAGCTCCCTAAAATCCAACAGTAATTTCTAAACTATCAT
GTGAAACATTGCCTTGTTTTTCGAATATTAAGGACGAGATTATAGTGCAAGAGAGCACCG
GTTTGAAAATGGATGACAATAGTTATTTCTCAAAGAGATTCACAGATCTTTCTCTTGTGA
TAGGTATGTGAAGCATAGAGTAACCAAAATTTGTTACAGGTTACTTACTTGCTTCGACGA
GCCTTTCTTTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 08-K112247-0022-270-D06C-8409
GenBank Accession LN489794 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K112247-0022-270-D06C-8409
GAAAAGAAAGGCTCGTCGAAGCAAGTAAGTAACCTGTAACAAATTTTGGTTACTCTATGC
TTCACATACCTATCACAAGAGAAAGATCTGTGAATCTCTTTGAGAAATAACTATTGTCAT
CCATTTTCAAACCGGTGCTCTCTTGCACTATAATCTCGTCCTTAATATTCGAAAAACAAG
GCAATGTTTCACATGATAGTTTAGAAATTACTGTTGGATTTTAGGGAGCTGCTTATGAAA
CTTATGCTGGTCTCTGTTCCAATGCAAATACGCAGATACATGTATGGGATGTCAGGAAAC
AACATCGGCTTGCAAATGCTTCTAGAAAAAGTCCCTCGGTCAAGGAAATGGCGATGTCTT
AGGAGCACGCAAGTGTGAAACAGATCCAATCCTTTCAAGCCAACAAAATCACTCTCCTGA
CTCTAGATTCGAAGCACATAATGTCAAATACTGATGTGGGATTGGCGAGGTAACATTTGT
CGGCCTTTCTTCAACTTGAATTGGATTCTCGTTCTTGGGTTGCAAAAAA

Last Updated on 06.12.2018 16:11