Sequences from confirmation amplicon


Confirmation sequences for line 270G02 and hit At5g16690 are shown.
Line ID 270G02
Locus At5g16690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5477536
Primers used for PCR PP05 - 8474, show primer details
   
Sequence Name 15-K102723-0022-270-G02C-8409
GenBank Accession LN492925 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K102723-0022-270-G02C-8409
GCCTTTACATTGAATATATCCTGGCTTAATAGATCAGCAATTAACTGTATGCATGCATAA
GGGGGAAATTTCACGTTTCTTCATTGCAACACAACTCACCTGAGCTTGCGCAAAACCCGT
CGGATAACATTATTACGTCCTGAATTGTGACTCTGAGAGTTAACAATTTCCGAAGGTGAA
AAGTATTTCAGGTAACGTGCTTCTGGGTCAAGTATCTCACAGAATATGTCTAGCAATCGA
AGTTTATCAAACTTTCCAGCTTCATATAAGCACTGCAAAACAATAGACAAAGAATCTGCT
CGTTAATTTATCCAGAAAGCACAAATCACTCTAATCAGATGAAAAACCGATCTGCCTCAT
ATTTCCTCGAGTGATGTATAATAGAAGAAGCATAATATGAATGCATCATGACTTGGGAGT
CCAACTAAACTGACAGAAAAGCTAATTCAATAGATACAACAAAAGAACTGCCTTCAATCT
AGAGATGAAAAAGAAACAGTGTATCTATAAAACTCAATATTCATCCAAGCGGAGAACAGA
AAGTGAAAGTTCCAAAATATTTCTCTGCCCCAATA
   
Sequence Name 31-K102728-0022-270-G02C-PP05
GenBank Accession LN500114 [GenBank]
Sequencing primer gene specific primer, PP05: 5'-TTATTGGGCAGAGAAATATTTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K102728-0022-270-G02C-PP05
TGAGTTTTATAGATACACTGTTTCTTTTTATCTCTAGCTTGAAGGCAGTTCTTTTGTTGT
ATCTATTGAATTAGCTTTTCTGTCAGTTTAGTTGGACTCCCAAGTCATGATGCATTCATA
TTATGCTTCTTCTATTATACATCACTCGAGGAAATATGAGGCAGATCGGTTTTTCATCTG
ATTAGAGTGATTTGTGCTTTCTGGATAAATTAACGAGCAGATTCTTTGTCTATTGTTTTG
CAGTGCTTATATGAAGCTGGAAAGTTTGATAAACTTCGATTGCTAGACATATTCTGTGAG
ATACTTGACCCAGAAGCACGTTACCTGAAATACTTTTCACCTTCGGAAATTGTTAACTCT
CAGAGTCACAATTCAGGACGTAATAATGTTATCCGACGGGTTTTGCGCAAGCTCAGGTGA
GTTGTGTTGCAATGAAGAAACGTGAAATTTCCCCCTTATGCATGCATACAATTAATTGCT
GATCTATTTAAGCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACATTCC

Last Updated on 06.12.2018 16:11