Sequences from confirmation amplicon


Confirmation sequences for line 271A05 and hit At5g37910 are shown.
Line ID 271A05
Locus At5g37910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15097183
Primers used for PCR CQ14 - 8474, show primer details
   
Sequence Name 64-K105459-0022-271-A05C-8409
GenBank Accession LN511203 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K105459-0022-271-A05C-8409
ATTTACATTGAATATGATGATCAATGCTTCACAGCAAATAGGACAATCGAGGATATCAAG
ATCTAGCAGTACTATCGCCGAGCGTTGTCTCTTAGCCACTCTCTCCCCTCCATCACCTTG
AGATATCAAAGCTTCCGAAATTGAAGCTAGCACCATGTCGCGTAGAAATCAATAGGAGAA
ATTTGTCCTCGGCTGATGAAGGGGGAAGGGTTTAGAGGGTTTGTTCAAAACTTTGATACA
AACAAAGGTGAGAATTCTCTTCTCGTGTCTGAACACCAGTTTATTTAATCTCAAAAAAAA
AGTATGATAATTAACTCTTAAACAAGAATGATGGAGAGATCTCTGTTTATAGAAGACGAA
TTTTAGAAAACACCTCTTAGAGTCTTAGGAATTGGAAATAAAATGTCAAAAATCCCAAAC
AGGAAAAAGGGAACAAACTAGAAAAAGGAAAAACAATCAAACAGAAGGGAAAGCT
   
Sequence Name 48-K105458-0022-271-A05C-CQ14
GenBank Accession LN506506 [GenBank]
Sequencing primer gene specific primer, CQ14: 5'-TAGCTTTCCCTTCTGTTTGATTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K105458-0022-271-A05C-CQ14
TTCCTTTTCTGTTTGGGATTTTTGACATTTTATTTCCATTCCTAAGACTCTAAGAGGTGT
TTTCTAAAATTCGTCTTCTATAAACAGAGATCTCTCCATCATTCTTGTTTAAGAGTTAAT
TATCATACTTTTTTTTTGAGATTAAATAAACTGGTGTTCAGACACGAGAAGAGAATTCTC
ACCTTTGTTTGTATCAAAGTTTTGAACAAACCCTCTAAACCCTTCCCCCTTCATCAGCCG
AGGACAAATTTCTCCTATTGATTTCTACGCGACATGGTGCTAGCTTCAATTTCGGAAGCT
TTGATATCTCAAGGTGATGGAGGGGAGAGAGTGGCTAAGAGACAACGCTCGGCGATAGTA
CTGCTAGATCTTGATATCCTCGATTGTCCTATTTGCTGTGAAGCATTGATCATCATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAG

Last Updated on 06.12.2018 16:11