Sequences from confirmation amplicon


Confirmation sequences for line 271A12 and hit At1g21920 are shown.
Line ID 271A12
Locus At1g21920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7705730
Primers used for PCR 32by - 8474, show primer details
   
Sequence Name 24-K108223-0022-271-A12C-32by
GenBank Accession LN497023 [GenBank]
Sequencing primer gene specific primer, 32by: 5'-CTAGCCAATCTCCTTCATAACGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K108223-0022-271-A12C-32by
CACTTCCGTTCACTTCCCTTTGTTAAATTCACCTTCGTAGAAATCTCCATTACTCCATAA
TTGAACTCCTTCTTTAACGAATCTCTTGATTACCTTCTTCTCCGGTTTTGAATCGTCTCC
GATGTACCATTGTACCGGTTTTGATTTCGAGTCTCTGTTTTGGAACCCTAAGTTTTTGAT
TGCGATTACGGTTTGGTTTAGTAGAGAGATGTTCTTGGAAGCGAAGAAGAGAGCTACAGC
GACGAAGATTAAAGCTAGTAAAAGATTCTCCGATGTGAGAATCTCATCGGTTTGTGAGAA
GACGACGAAGAAGAAGAACGATGAGAGACTTAGGAAAGAGAGAGATACCATTGTGAAAGC
TAAACCGGGATTGATTCTGGTTAAACCGGTTTTAGGATTTGGTGAACCGAATGGTTTAGG
TGGTTTTCTTCTCTGTTGAATATATGGTTTCATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 08-K108222-0022-271-A12C-8409
GenBank Accession LN489765 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K108222-0022-271-A12C-8409
ATTTACAATTGAATGAAACCATATATTCAACAGAGAAGAAAACCACCTAAACCATTCGGT
TCACCAAATCCTAAAACCGGTTTAACCAGAATCAATCCCGGTTTAGCTTTCACAATGGTA
TCTCTCTCTTTCCTAAGTCTCTCATCGTTCTTCTTCTTCGTCGTCTTCTCACAAACCGAT
GAGATTCTCACATCGGAGAATCTTTTACTAGCTTTAATCTTCGTCGCTGTAGCTCTCTTC
TTCGCTTCCAAGAACATCTCTCTACTAAACCAAACCGTAATCGCAATCAAAAACTTAGGG
TTCCAAAACAGAGACTCGAAATCAAAACCGGTACAATGGTACATCGGAGACGATTCAAAA
CCGGAGAAGAAGGTAATCAAGAGATTCGTTAAAGAAGGAGTTCAATTCTACAGTAATGGA
GATTTCTACGAAGGTGAATTTAACAAAGGGAAGTGTAACGGAAGTGGTGTGTATTACTAT
TTCGTGAGAGGACGTTATGAAGGAGATTGGCTA

Last Updated on 06.12.2018 16:11