Sequences from confirmation amplicon


Confirmation sequences for line 272G02 and hit At1g69580 are shown.
Line ID 272G02
Locus At1g69580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26173077
Primers used for PCR PP26 - 8474, show primer details
   
Sequence Name 46-K103008-0022-272-G02C-8409
GenBank Accession LN505833 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K103008-0022-272-G02C-8409
GCCTTTACATTGAACCTGAAACCAAAACATTTTAATACCCCAAAAGAGACATTATTGTGC
TTAATTGTAGTTAATTAGGAGGTTCTGGTTGATGTTATTACTTCGATTTGTTCATGAAGT
TTCTTCTGAACTTCCATCTGCATTTGTAAAGCCTCTGTGATTTGTAGCCCTCTGCTTCAA
ATGAACAAACAATTAATTTTCATCTTTATCTAAAATCTATCTATAAAAGACTATTTGATT
TTCTGATCATTACTCTTTAGCTGGATTGCTGTTTTCTTCGGTGACACTGCAGCCTCGGAG
ATCTCTTGAATCGTTTTTACTCTCAACTTCTTGATTCTCTGATGCAGAGGAAACTGCTCA
TCAAGAAACAAAACATATTTTAACCTAATTTGGTCAGATCAGAGTCCAAACTCAAGAATT
TGTTTCTATCTTTTCTCTAAAAACATTATCAATGTTAATATTTGATCAGAAAACATCAAA
CCTTCTAGCTTGTTATCATCGAACTTCATGCTCTTCCCTAACCGATATTTCTGCAGAAAG
ATAGAATAAATTAGATTTTCAAGAGACAATAGAAAAAAAGACTCATCTAATTAAAGATTC
TGGTGAATACTGATCAAACCTGTAAATGGCTCTTGAGATGGTATAAGGTAAGCCCAGGAA
TCTCCATAACCTTCATCAAACCCTTAGGTGTTGCTTCTACAAACATAAACAGAACAACCA
AATATATTAAAAAAAGACAATCAAGAAACATGAAAATGAAGATGAAAAAAAGACAAAAGT
ACGTACTGTTAGGTCCTCCAAGTTGATTAACGGCTTCGATGAATTTGTGATGAAGATCAC
AAGTCCATTTCAACCTTGGCTTAGCATCCGTTGACAACACAAGGCTCATCTTTGCCTTGT
GATTATCCATAGTAGTATTAGTATTGTTGGCATTATTGTTGATCTCCCTTAATAAA
   
Sequence Name 94-K103009-0022-272-G02C-PP26
GenBank Accession LN515302 [GenBank]
Sequencing primer gene specific primer, PP26: 5'-ACAAACCCAACAATCTTTGAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K103009-0022-272-G02C-PP26
TCTTGGGTCGTTTCTAAATTTGTATGTGTTTATTAATGGAGATCAACAATAATGCCAACA
ATACTAATACTACTATTGATAATCACAAGGCAAAGATGAGCCTTGTGTTGTCAACGGATG
CTAAGCCAAGGTTGAAATGGACTTGTGATCTTCATCACAAATTCATCGAAGCCGTTAATC
AACTTGGAGGACCTAACAGTACGTACTTTTGTCTTTTTTTCATCTTCATTTTCATGTTTC
TTGATTGTCTTTTTTTAATATATTTGGTTGTTCTGTTTATGTTTGTAGAAGCAACACCTA
AGGGTTTGATGAAGGTTATGGAGATTCCTGGGCTTACCTTATACCATCTCAAGAGCCATT
TACAGGTTTGATCAGTATTCACCAGAATCTTTAATTAGATGAGTCTTTTTTTCTATTGTC
TCTTGAAAATCTAATTTATTCTATCTTTCTGCAGAAATATCGGTTAGGGAAGAGCATGAA
GTTCGATGATAACAAGCTAGAAGGTTTGATGTTTTCTGATCAAATATTAACATTGATAAT
GTTTTTAGAGAAAAGATAGAAACAAATTCTTGAGTTTGGACTCTGATCTGACCAAATTAG
GTTAAAATATGTTTTGTTTCTTGATGAGCAGTTTCCTCTGCATCAGAGAATCAAGAAGTT
GAGAGTAAAAACGATTCAAGAGATCTCCGAGGCTGCAGTGTCACCGAAGAAAACAGCAAT
CCAGCTAAAGAGTAATGATCAGAAAATCAAATAGTCTTTTATAGATAGATTTTAGATAAA
GATGAAAATTAATTGTTTGTTCATTTGAAGCAGAGGGCTACAAATCACAGAGGCTTTACA
AATGCAGATGGAAGTTCAGAAGAAACTTCATGAACAAATCGAAGTAATAACATCAACC

Last Updated on 06.12.2018 16:11