Sequences from confirmation amplicon


Confirmation sequences for line 273B10 and hit At4g32000 are shown.
Line ID 273B10
Locus At4g32000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15475657
Primers used for PCR 83yl - 8474, show primer details
   
Sequence Name 68-K120400-0022-273-B10C-83yl
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 83yl: 5'-GACGGTAATCTTATAGGACGAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K120400-0022-273-B10C-83yl
CACACACTCTAGCAGCAGTCAAAAGATCGAAAACGTTAGTCAAGAAGCAAAACGAGAATT
TCAGGTACCAAAACAAAACACAAGTACTAATCATTTTTACTTTTTTACCGGATTCTTGAT
ATCTGATTTTATCATCTAAATCTCAGAATGAAGTTGATTTGTTGATTTTATCAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TC
   
Sequence Name 44-K120399-0022-273-B10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K120399-0022-273-B10C-8409
ACATTGAATATATCCTGATAAAATCAACAAATCAACTTCATTCTGAGATTTAGATGATAA
AATCAGATATCAAGAATCCGGTAAAAAAGTAAAAATGATTAGTACTTGTGTTTTGTTTTG
GTACCTGAAATTCTCGTTTTGCTTCTTGACTAACGTTTTCGATCTTTTTGACTGCTGCTA
GAGTGTTGTTGCCTAAACAGGCCTTGTAAACATCTCCGAACCCGCCTCGTCCTATAGAAT
TACCGT

Last Updated on 06.12.2018 16:11