Sequences from confirmation amplicon


Confirmation sequences for line 273D03 and hit At4g39050 are shown.
Line ID 273D03
Locus At4g39050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18200078
Primers used for PCR 13dr - 8474, show primer details
   
Sequence Name 32-K110172-0022-273-D03C-13dr
GenBank Accession LN500625 [GenBank]
Sequencing primer gene specific primer, 13dr: 5'-CCAAAATATTTAGCAACAGGCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K110172-0022-273-D03C-13dr
ATATCTGCAAGTTATCAATATTTACATCTTTTTTTTAGTCTTATCTGATCTGTTTTAACA
TCTGATATTGATCATAATATTAGTGTTTAATCGGGTTGACACTAAGAATAAATCTAACCA
ATAATTAATCCAAACACTGAACCTAAAAAGCTTAAAGAATTGGGTAAGTCAGCTTTTGTT
TTCTACATATCAAAAACCAAACTTTAACTTTGAGTTTCTCATATACAGTTGTGATAGACA
ATCCAAACTCAGTTTTTAGCTTCAATTTGATTTCTACATTTACTAAACCTAAAATGGAAT
GTAACATAAAAGACGCATCCACTTGCTAGAAAGGTTACCTTTTCAACTGGTTAAGACGGA
AACGCAAAGAGCCGATCAGAGATCTTTGTGCGGCAAATTGGGCATTCAGAGCAAGCAAGG
GGGCTCTGTCACGTCATTGTGCGGCAAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCACGGTTAT
   
Sequence Name 16-K110171-0022-273-D03C-8409
GenBank Accession LN493473 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K110171-0022-273-D03C-8409
CCATTTACAATTTGCCGCACAATGACGTGACAGAGCCCCCTTGCTTGCTCTGAATGCCCA
ATTTGCCGCACAAAGATCTCTGATCGGCTCTTTGCGTTTCCGTCTTAACCAGTTGAAAAG
GTAACCTTTCTAGCAAGTGGATGCGTCTTTTATGTTACATTCCATTTTAGGTTTAGTAAA
TGTAGAAATCAAATTGAAGCTAAAAACTGAGTTTGGATTGTCTATCACAACTGTATATGA
GAAACTCAAAGTTAAAGTTTGGTTTTTGATATGTAGAAAACAAAAGCTGACTTACCCAAT
TCTTTAAGCTTTTTAGGTTCAGTGTTTGGATTAATTATTGGTTAGATTTATTCTTAGTGT
CAACCCGATTAAACACTAATATTATGATCAATATCAGATGTTAAAACAGATCAGATAAGA
CTTAAAAAAAAGATGTAAATATTGATTAACTTGCATGATTATGCAGGTCTCTAAGAACTG
TGGGTGAAGCCTGTTGCTAAATATTTTGG

Last Updated on 06.12.2018 16:11