Sequences from confirmation amplicon


Confirmation sequences for line 273D11 and hit At5g64570 are shown.
Line ID 273D11
Locus At5g64570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25812814
Primers used for PCR CL23 - 8474, show primer details
   
Sequence Name 30-K028746-022-273-D11C-8409
GenBank Accession LN499590 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K028746-022-273-D11C-8409
TAAAATTTTCGAAAACAAAAATCAAAAGTGGATAAAAAAATTAATTACCTTCGCCAATGG
CTTCGAAACAGAGATACGTTGAAAGAAGCGGCGGTGAGTATAACCTGCGGGAAACTCGTA
GCTCCGGGAACTTGGCTAGAAAAATGCGTGCCGGGTCCGATGTAAGAAACGCCGTGAAGT
GCTTCAGACCACCATTCATACGTTGGAATCCCAAGACGAGTCACGCCGTTAGCTTTACTC
ACTAAAAACCCGATCTTTTCTTGCAACGTGAGCCTCGCGACCAGATCAGCGACTCGGTAT
TCGATCTTCAAAACGGTGTTGCAGAAACCATAGGCGGCGAGAGAAGGGTTTGCAGCTACA
TCGCAGGCGAAAACCGGCGATGATTGAGCGTTTGAGAAATTTAAAAAGTAGAGGAAGAAA
CAGAGAAAGATTATATAAACAGAGGA
   
Sequence Name 30-K028746-022-273-D11C-CL23
GenBank Accession LN499591 [GenBank]
Sequencing primer gene specific primer, CL23: 5'-ACCATCCTCTGTTTCCTCTGTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K028746-022-273-D11C-CL23
CTTTTAATTTCTCAAACGCTCAATCATCGCCGGGTTTTCGCCTGCGACGTAGCTGCAAAC
CCTTCTCTCGCCGCTNCTATGGTTTCTGCAACACCNGTTTTGAAGATCGAATACCGAGTC
GCTGATCTGGTCGCGAGGCTCACGTTGCAAGAAAAGATCGGGTTTTTAGTGAGTAAAGCT
AACGGCGTGACTCGTCTTGGGATTCCAACGTATGAATGGTGGTCTGAAGCACTTCACGGC
GTTTCTTACATCGGACCCGGCACGCATTTTTCTAGCCAAGTTCCCGGAGCTACGAGTTTC
CCGCAGGTTATACTCACCGCCGCTTCTTTCAACGTATCTCTGTTTCAAGCCATTGGCAAG
GTAATTAATTTTTTATCCACTTTTGATTTTTGTTTTCAAAATTTTATTTTCGTCCTTTGC
TTTTATTCTCTTTTTGGATAATGGGAATATCTTACTTATTATGAAACTGGGCCTCTCCTT
TTTTTTTGATCTTTCGTTTGGTACAAGTAAACACTTTACAACACGTTATACTACGTCGTT
TTCGTAGATGGATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACAATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11