Sequences from confirmation amplicon


Confirmation sequences for line 274A02 and hit At4g22120 are shown.
Line ID 274A02
Locus At4g22120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11717251
Primers used for PCR KR45 - 8474, show primer details
   
Sequence Name 80-K100822-0022-274-A02C-8409
GenBank Accession LN513913 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K100822-0022-274-A02C-8409
GCCATTTACACTGAAAAATTACTAATGATGGACTGACTTGTTTGGTTTAATGCAGTAAAT
TCATGAAATCGGTGATACAAGGTTTCCTTCCGGGTATTGCACTGAAGCTTTTCCTCGCCT
TTCTGCCATCCATTTTGATGATCATGTCCAAATTTGAAGGCTTCACATCGATCTCATCTT
TAGAGAGACGAGCAGCGTTTCGATATTACATCTTCAACTTAGTGAACGTCTTTCTTGCTA
GCGTTATCGCTGGAGCTGCGTTTGAACAGCTTAACTCTTTCCTCAATCAATCCGCAAACC
AAATCCCCAAAACCATTGGTGTGGCGATACCGATGAAAGCAACTTTCTTCATCACGTATA
TAATGGTTGATGGTTGGGCAGGAGTTGCAGGAGAGATTCTAATGCTGAAACCATTGATTA
TGTTCCATCTCAAAAACGCCTTCTTGGTTAGAACTGATA
   
Sequence Name 80-K100834-0022-274-A02C-KR45
GenBank Accession LN513915 [GenBank]
Sequencing primer gene specific primer, KR45: 5'-TATCAGTCTTAACCAAGAAGGCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K100834-0022-274-A02C-KR45
GTTTCAGCATAGAATCTCTCCTGCAACTCCTGCCCACCATCAACCATTATATACGTGATG
AAGAAAGTTGCTTTCATCGGTATCGCCACACCAATGGTTTTGGGGATTTGGTTTGCGGAT
TGATTGAGGAAAGAGTTAAGCTGTTCAAACGCAGCTCCAGCGATAACGCTAGCAAGAAAG
ACGTTCACTAAGTTGAAGATGTAATATCGAAACGCTGCTCGTCTCTCTNAAGATGAGATC
GATGTGAAGCCTTCAAATTTGGACATGATCATCAAAATGGATGGCAGAAAGGCGAGGAAA
AGCTTCAGTGCAATACCCGGAAGGAAACCTTGTATCACCGATTTCATGAATTTACTGCAT
TAAACCAAACAAGTCAGTCCATCANTAGTAATTTTTTCAGTTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11