Sequences from confirmation amplicon


Confirmation sequences for line 274B08 and hit At3g10210 are shown.
Line ID 274B08
Locus At3g10210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3160535
Primers used for PCR CK41 - 8474, show primer details
   
Sequence Name 12-K018063-022-274-B08C-CK41
GenBank Accession LN491360 [GenBank]
Sequencing primer gene specific primer, CK41: 5'-CAACTTGTTTATTTCATCCACCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K018063-022-274-B08C-CK41
TCATTGCCACTCTTGGCCGCCTTCTTTTAAGTGGAGGGTAATCATTTCTTTTCATATGTA
TCAGTTGATTTATATACAAGTTATTTCAAACTTGCATAATCGAATATTGAACCCTAAAGT
TTGAAGCTTTCATGATCTAATAAACTCTGGCGTCTAGGAGTATTGATCTTCTTTTGAGCT
TGTAACTTGAAACGGTTATGGGATCTAGATTACTTTAGAATCAACAACTGTGGACTTAAC
TGTTTTGTCAAAACAATATCTCTTTCAACATTGCAGACTGTACTGGAAGATTAAGTACGT
GAGCAGGTTGCAGTACCTTTGGGAGGACATAAAGAAAGGCGAGGTGGAAATTCCCGACTT
TGTGAAGAACCATGACATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACATTCACG
   
Sequence Name 16-K018063-022-274-B08C-8409
GenBank Accession LN493222 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K018063-022-274-B08C-8409
ATGAAGCCATTTACAATTGAATATATCATGTCATGGTTCTTCACAAAGTCGGGAATTTCC
ACCTCGCCTTTCTTTATGTCCTCCCAAAGGTACTGCAACCTGCTCACGTACTTAATCTTC
CAGTACAGTCTGCAATGTTGAAAGAGATATTGTTTTGACAAAACAGTTAAGTCCACAGTT
GTTGATTCTAAAGTAATCTAGATCCCATAACCGTTTCAAGTTACAAGCTCAAAAGAAGAT
CAATACTCCTAGACGCCAGAGTTTATTAGATCATGAAAGCTTCAAACTTTAGGGTTCAAT
ATTCGATTATGCAAGTTTGAAATAACTTGTATATAAATCAACTGATACATATGAAAAGAA
ATGATTACCCTCCACTTAAAAGAAGGCGGCCAAGAGTGGCAATGACAAGTCTTGAACGAA
GACCAGGGTGGATGAAAT

Last Updated on 06.12.2018 16:11