Sequences from confirmation amplicon


Confirmation sequences for line 274C02 and hit At2g35020 are shown.
Line ID 274C02
Locus At2g35020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14757753
Primers used for PCR 78cs - 8474, show primer details
   
Sequence Name 52-K108895-0022-274-C02C-78cs
GenBank Accession LN507842 [GenBank]
Sequencing primer gene specific primer, 78cs: 5'-ACTTCCGTTCATTGAGTTCTGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K108895-0022-274-C02C-78cs
CGGCATAGAACCGGTGCCGGAGAATTGTGTGTCAACGGTGGAGGAAAGAACTAAGGAAGA
CAGAGAAAAATGGTGGAAAATGGGATTAAAAGCTATCTACGAAGGCAAATTGGGTGTGGT
GCTTTTATCTGGTGGACAGGTACTTTTAATTATTGATGCTTGGGGTGTTATGTTGCTTTG
GAAACAAAACATTGATCTGGCTCTGAGTCCTTGTCTTGGATTACTTCTATGTAGCTTACT
AAAGATTGTTGAGAAGATGTTTAATGATTCTAGGATTATGGTTGGAATTTGGGGGGTGAT
TGCTGAAATGATGTTGGAATAATGCGTGGTACCTAATTCAAATAATGCATTTATAGATGT
CCGTTACTTAAATAATTGCTAAGTCTGAAAAGTGAAAAGTACTTATTTTGATAATTGTGG
TCATAATTGTGGTTATTTTGATAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 36-K108894-0022-274-C02C-8409
GenBank Accession LN502254 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K108894-0022-274-C02C-8409
TTATCAAAATACCACAATTATGACCACAATTATCAAAATAAGTACTTTTCACTTTTCAGA
CTTAGCAATTATTTAAGTAACGGACATCTATAAATGCATTATTTGAATTAGGTACCACGC
ATTATTCCAACATCATTTCAGCAATCACCCCCCAAATTCCAACCATAATCCTAGAATCAT
TAAACATCTTCTCAACAATCTTTAGTAAGCTACATAGAAGTAATCCAAGACAAGGACTCA
GAGCCAGATCAATGTTTTGTTTCCAAAGCAACATAACACCCCAAGCATCAATAATTAAAA
GTACCTGTCCACCAGATAAAAGCACCACACCCAATTTGCCTTCGTAGATAGCTTTTAATC
CCATTTTCCACCATTTTTCTCTGTCTTCCTTAGTTCTTTCCTCCACCGTTGACACACAAT
TCTCCGGCACCGGTTCTATTGCCGCCACTGGCAACCCTAATGAAGTGAAAAACAGAACTC
AATGAACGGAAG

Last Updated on 06.12.2018 16:11