Sequences from confirmation amplicon


Confirmation sequences for line 274C12 and hit At2g05180 are shown.
Line ID 274C12
Locus At2g05180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1875829
Primers used for PCR ZZ61 - 8474, show primer details
   
Sequence Name 77-K021646-022-274-C12C-8409
GenBank Accession LN513470 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K021646-022-274-C12C-8409
TGAGCCATTTAAATTGAATATATCCGGAGCAGCTTAGTGGCGATTAGCTTCTTCATGAAC
TTCCAGTAATCTCCATAAGGAGCGGTAATGATGCCAGAAGATCCAAACAGGAGGGACTCA
TCGATTGCAGCAACACCGCGAGAGGAGACGTTCACGTCATGAGCCCTGAAGATCTCGTAG
GCAACAGAGGCTGAAGAGACGAGAATGACTGGGACATTAAAGATCCGTAGATGGAGTAGA
TGTCCATACTTAGAGGAGAGTTTCTGAAGAGACTTGTGGGTTAGAGGAGAGAAGAGAAGG
TGAAGATGACCGATGATTGGAAGAGAAGGAGGGCTAGGAGGAAGATCACAGCCTCGCGGG
TGCTTTGGTTTCTTGAAGAAGAGAGAGTAACAGAAGAGTGAGAAGAGGCATAGGACGATG
AAGATGAAACAGTTCTGAAAGTCAACGCTGAGAATTGCTGCCATTGTTGCTTAAAGTGAA
GAGGG
   
Sequence Name 78-K021646-022-274-C12C-ZZ61
GenBank Accession LN513615 [GenBank]
Sequencing primer gene specific primer, ZZ61: 5'-ATTATGTTTCAGCTCCCTCTTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K021646-022-274-C12C-ZZ61
GACATTCTCAGCGTTGACTTTCAGAACTGTTTCATCTTCATCGTCCTATGCCTCTTCTCA
CTCTTCTGTTACTCTCTCTTCTTCAAGAAACCAAAGCACCCGCGAGGCTGTGATCTTCCT
CCTAGCCCTCCTTCTCTTCCAATCATCGGTCATCTTCACCTTCTCTTCTCTCCTCTAACC
CACAAGTCTCTTCAGAAACTCTCCTCTAAGTATGGACATCTACTCCATCTACGGATCTTT
AATGTCCCAGTCATTCTCGTCTCTTCAGCCTCTGTTGCCTACGAGATCTTCAGGGCTCAT
GACGTGAACGTCTCCTCTCGCGGTGTTGCTGCAATCGATGAGTCCCTCCTGTTTGGATCT
TCTGGCATCATTACCGCTCCTTATGGAGATTACTGGAAGTTCATGAAGAAGCTAATCGCC
ACTAAGCTGCTCCGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATTTCGACGTGTCTACT

Last Updated on 06.12.2018 16:11