Sequences from confirmation amplicon


Confirmation sequences for line 274F06 and hit At3g60270 are shown.
Line ID 274F06
Locus At3g60270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22278163
Primers used for PCR KR42 - 8474, show primer details
   
Sequence Name 68-K100822-0022-274-F06C-8409
GenBank Accession LN511957 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K100822-0022-274-F06C-8409
TCGGACTGAGCCTTTACAATTGAATATATCCTTCCGTCTCCATCAGCTCCATCGCCGTCT
CCATCGCCGGGAAACGCAGAAAACTTGAAAAATGCGGCGTCTAAGGGAATCATGAGCTAT
GGAAAGATTGTGGTGACGATGGTGTTGATGTACGGTGTGCTTAATTAGATATTCCCGCAA
CAATTGCCTCGATCTATTTTTGGTCTTGGTCTTTCGAACTAGTCCATCATGAAGTGCTCT
TCTTGTGTGTAATAAATTATTTTCTTCATCAATACTTTTTTGCTTATTGTTATCTTTACT
CGATCTCTTTTGTTCTATTACTCATGGTCGTAGCACAGCGTTATCGTTATCATGTTCTAC
ATGCTTATTGAGTACAGTACAAATTAATCTTAGATTCCGGTTCCTTCCTCTTGGAACAAT
ATTTACCGTTCGAATTTTGGGACATAAGGAAATACAAGTTGTCTTATATCTNAGCCAAAT
TAGAGCGT
   
Sequence Name 68-K100834-0022-274-F06C-KR42
GenBank Accession LN511959 [GenBank]
Sequencing primer gene specific primer, KR42: 5'-TTGACGCTCTAATTTGGCTTAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K100834-0022-274-F06C-KR42
AAATTCGAACGGTAAATATTGTTCCAGAGGAAGGAACCGGAATCTAAGATTAATTTGTAC
TGTACTCAATAAGCATGTAGAACATGATAACGATAACGCTGTGCTACGACCATGAGTAAT
AGAACAAAAGAGATCGAGTAAAGATAACAATAAGCAAAAAAGTATTGATGAAGAAAATAA
TTTATTACACACAAGAAGAGCACTTCATGATGGACTAGTTCGAAAGACCAAGACCAAAAA
TAGATCGAGGCAATTGTTGCGGGAATATCTAATTAAGCACACCGTACATCAACACCATCG
TCACCACAATCTTTCCATAGCTCATGATTCCCTTAGACGCCGCANTTTTCAAGTTTTCTG
CGTTTCCCGGCGATGGAGACGGCGATGGAGCTGATGGAGACGGAAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT

Last Updated on 06.12.2018 16:11