Sequences from confirmation amplicon


Confirmation sequences for line 274F12 and hit At2g13370 are shown.
Line ID 274F12
Locus At2g13370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:5544454
Primers used for PCR 41gz - 8474, show primer details
   
Sequence Name 34-K112586-0022-274-F12C-41gz
GenBank Accession LN501473 [GenBank]
Sequencing primer gene specific primer, 41gz: 5'-TCTCAGATAACCATTTTAATGCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K112586-0022-274-F12C-41gz
GATGACATGAGATTGTGGAACTATGTTTCAACTTTCTCAAATTTGTCGGGGGATCGACTC
AATCAAATCTACTCCAAATTGAAGCAGGAGAAAGAGGAAGAGGAAGGGGTCGGGCCATCG
CACCTCAATGGTTCTAGGAACTTCCAGAGGCAACAAAAATTCAAAACAGCAGGGAACTCC
CAAGGATCACAGCAGGTTCACAAGGGCATTGACACAGCAAAGTTTGAAGCGTGGAAACGA
AGAAGAAGAACAGAAAACGATGTTCAGACTGAGCGACCAACGATAACAAACTCTAACTCG
CTGGGAATACTCGGCCCTGGACCATTGGATAGAAGCCATAGGGCACGTCAAACCGGATTT
CCCCCAAGATAAGTTTTCTCTGTTCTGGTACCATACATTATGCTCTGCATTTGCCGGTTG
AACTCTGGTTTGATCATCGATTCAGAAGCTCAACAACATAGATACCCGGAAAACTCATTT
TTTTTAAAAAGTCGGAAACACGGCAGCAGTGTTCGGTTTTGGTTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGNTTATT
   
Sequence Name 08-K112585-0022-274-F12C-8409
GenBank Accession LN489799 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K112585-0022-274-F12C-8409
TTCATTGACCAAACCGAACACTGCTGCCGTGTTTCCGACTTTTTAAAAAAAATGAGTTTT
CCGGGTATCTATGTTGTTGAGCTTCTGAATCGATGATCAAACCAGAGTTCAACCGGCAAA
TGCAGAGCATAATGTATGGTACCAGAACAGAGAAAACTTATCTTGGGGGAAATCCGGTTT
GACGTGCCCTATGGCTTCTATCCATGGTCCAGGGCCGAGTATTCCCAGCGAGTTAGAGTT
TGTTATCGTTGGTCGCTCAGTCTGAACATCGTTTTCTGGCTTCTTCTTCGTTTCCACGCT
TCAAACTTTGCTGTGTCAATGCCCTTGTGAACCTGCTGTGATCCTTGGGAGTTCCCTGCT
GTTTTGAATTTTTGTTGCCTCTGGAAGTTCCANACCATTGAGGTGCGATGGCCCGACCCC
TTCCTCTTCCTCTTTCTCCTGCTTCAATTTGGAGTAGATTTGATTGAGTCGATCCCCCGA
CAATTTGAGAAAGTTGAAACATAGTTCCACAATCTCATTNCATTCCTGCATTTTGATTTC
CCGTTAATGATACAACCATGGGCATTAAAATGGTATCTGAGA

Last Updated on 06.12.2018 16:11