Sequences from confirmation amplicon


Confirmation sequences for line 274G02 and hit At4g12880 are shown.
Line ID 274G02
Locus At4g12880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7544960
Primers used for PCR PP25 - 8474, show primer details
   
Sequence Name 47-K103008-0022-274-G02C-8409
GenBank Accession LN506169 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K103008-0022-274-G02C-8409
TTACATTGGACAAGATTTAAAGCTAAACCAATTTCAAATCAGATCTAAATATGACAGATC
TGAGAGTGTAGATTGAGAAAGAAGAGATAGAGAGAACGTACAAAGCCAGTCTCCGACGTA
GAAATGTTTGCCTTGAGCCCATAGGGTATAGTTAATGTTGGGGTTCCAGAACTTTTTGTC
GCCGACTAAATATTTCTTGGCTGTTACTTCTGGTATCGGCGCCGCCACTAGAAAAGCCAG
TACAACCGCCGAGATTAGCACCATAGATCTCCCCATTTCTCTCTAGCAAAACTCTCTCTC
TTTCTCCCTTGTAGAATTAATTAGCTATCATAAATATAGTAGTTCATCAGTTCCACTTCC
ACTAAATTATTGTTTTTGGCAAAACAGTAACTTAAGTTATATAAAAAAAAAAATCATTAG
TCAATCAATCACAGTCCTTTATGATAAAACGAACTCATAATTATTCCCCCGACAACATGC
GTTTTAAATTATTTTTTCTTAAATTATATAATATTATATTGATATCAACCTAGCTAAAAT
AATTCGGATGGCGAAATCGGACAATTTTTAATAAAAAAAATGGGTATGAAAATAGTCTAT
GATTCCGTTCTT
   
Sequence Name 95-K103009-0022-274-G02C-PP25
GenBank Accession LN515348 [GenBank]
Sequencing primer gene specific primer, PP25: 5'-GTCGCTAAGAACGGAATCATAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K103009-0022-274-G02C-PP25
AATTGTCCGATTTCCCATCCGAATTATTTTAGCTAGGTTGATATCAATATAATATAATAT
AATTTAAGAAAAAATAATTTAAAACGCATGTTGTCGGTGGAATAATTATGAGTTCGTTTT
ATCATAAAGGACTGTGATTGATTGACTAATGATTTTTTTTTTTATATAACTTAAGTTACT
GTTTTGCCAAAAACAATAATTTAGTGGAAGTGGAACTGATGAACTACTATTTTTATGATA
GCTAATTAATTCTACAAGGGAAAAAAAAAGAAAGTTTTGCTAAAAAGAAATGGGGAAATC
TATGGGGCTAATCTCGGCGGTTGTACTGGCTTTTCTAGTGGCGGCCCCAATCCCAAAAGT
AACACCCAAAAAATATTTAGTCGGCGACAAAAAGTTCTGGAACCCCAACATTAACTATAC
CCTATGGGCTCAAGGCAAACATTTCTACTTCGGAAACGGGCTTTGTACGTTCTCTCTATC
TCTTCTTTCTCAATCTACACTCTCAAATCTGTCAAATTTAAATCTGATTTGAAATTGGTT
TACCTTTAAATCTTGTCCAATTGTAAATGGCTTCATGTCCGGGAAATCTACTTGGATCAC
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAAATTTCCGCACCGTTTTTATAAAATGAAAGTACTTTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATGGGCAAAAGCAATAAACAAATTATTCTAATTCGAAAA
TCTTTATTTCGACGTGTC

Last Updated on 06.12.2018 16:11