Sequences from confirmation amplicon


Confirmation sequences for line 274H04 and hit At4g33250 are shown.
Line ID 274H04
Locus At4g33250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16039271
Primers used for PCR CL78 - 8474, show primer details
   
Sequence Name 80-K033098-022-274-H04C-8409
GenBank Accession LN513895 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K033098-022-274-H04C-8409
TGTCTTTATTGACAATAAAAGGTCACAAATTAGATTTCCAGAACTATGATGTGGAATATT
CGTAACAGAAAATTATATGAGAAAGTAAAAGATTTCACATCCGAAGTTCTACTATTTTGA
TGACTTAAGCTATCCGTGGTGAAAACGAGATCATAAACCAAACATCATACCTCAGCCAGC
ACTGATCTTGGAACCTTTTGGTAGCTTAAGCTAAGAAGGTGACTTGCATATGCTTGAATA
GCTTGCTCAAAACCTGCATTTAATCTCTCGCATATAAGACATCTACACTAGACACAATCA
TAAAATGAATAGACAGTACCATAAACCACTACTGAGGACTTTGTTCGTACCATAAGACAT
CTACACACAAATATCCTTAAGTAACAAATCTACATCTAGAAATTTGTTATTAGTAGACGA
TTTATTTATGATTCAGAAGTTAGTTACCTGGAACAGCCTCGAGAATGTGACGGTTCTTGG
CAGCTTCATCCCAGAACTGTTGGAACCTCCCAGTCTGCAAAAGAAAAACAAGTGCTTTAA
ATACTCAATGCACACCTTTTCAACTCTCAACTAACTAATACTGATGTTTGCGTACCTCAA
GGTAGTGTGATAGAACAATTAGTGACTTGAACTGCTCCTCCATTTGCTGAAACACACAAT
CCCAACAAGTACATTTATTAGTCCTCAAGAATCCAACACATTGAAACTCACACTTTATCA
T
   
Sequence Name 32-K033098-022-274-H04C-CL78
GenBank Accession LN500477 [GenBank]
Sequencing primer gene specific primer, CL78: 5'-ATGCCATTCCAAGTTATTTCTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K033098-022-274-H04C-CL78
GATAAGTGTGAGTTTCATGTGTTGGATTCTTGAGGACTAATAAATGTACTTGTTTGGATT
GTGTGTTTCAGCAAATGGAGGAGCAGTTCAAGTCACTAATTGTTCTATCACACTACCTTG
AGGTACGCAAACATCAGTATTAGTTAGTTGAGAGTTGAAAAGGTGTGCATTGAGTATTTA
AAGCACTTGTTTTTCTTTTGCAGACTGGGAGGTTCCAACAGTTCTGGGATGAAGCTGCCA
AGAACCGTCACATTCTCGAGGCTGTTCCAGGTAACTAACTTCTGAATCATAAATAAATCG
TCTACTAATAACAAATTTCTAGATGTAGATTTGTTACTTAAGGATATTTGTGTGTAGATG
TCTTATGGTACGAACAAAGTCCTCAGTAGTGGTTTATGGTACTGTCTATTCATTTTATGA
TTGTGTCTAGTGTAGATGTCTTATATGCGAGAGATTAAATGCAGGTTTTGAGCAAGCTAT
TCAAGCATATGCAAGTCACCTTCTTAGCTTAAGCTACCAAAAGGTTCCAAGATCAGTGCT
GGCTGAGGTATGATGTTTGGTTTATGATCTCGTTTTCACCACGGATAGCTTAAGTCATCA
AAATAGTAGAACTTCGGATGTGAAATCTTTTACTTTCTCATATAATTTTCTGTTACGAAT
ATTCCACATCATAGTTCTGGAAATCTAANTTGTTGACCTTTTATTTGTCAATAAATGACA
ATTTTCACAGGCTGTTAACATGGATCAGCAATG

Last Updated on 06.12.2018 16:11