Sequences from confirmation amplicon


Confirmation sequences for line 275D11 and hit At1g26970 are shown.
Line ID 275D11
Locus At1g26970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9361152
Primers used for PCR DL75 - 8474, show primer details
   
Sequence Name 30-K018601-022-275-D11C-DL75
GenBank Accession LN499523 [GenBank]
Sequencing primer gene specific primer, DL75: 5'-ACAAGCACACAGCTATATGTCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K018601-022-275-D11C-DL75
ACAAAAAAAAATGCATGCACTCATAAAACCTAACTATCTCTGTCGCTACTTCACCGAACA
CGTCGACATCGTCTACTAGACAACACCGGATCAGCAACCGGAGTTCTTACTCTCTGTTTC
GCCGTGAAAGAAGAGGAAGAAGATGTCAATTTCATAACCGAGTTTGAAATAGAACCCGAC
TTCAACGTCATCTCCAACTCTTCAAGAGTAGATAACACATCAGACATCTTTGGCCTCAAC
TTAGGTTCTTGATTAAGACATTGCAACGCAGTATTAGCAGTCAAACAAGCTCCTTTGTGA
GGATATTGTCCCCCAAGCTTTGTGTCCATTATCCTAAACACTTTCCTTTTGTCTCCTAAG
TAAGGTATTGCCCAATCAACCAGATTTCGTTCCACTCCTACTTTCGTTTTGTCTACGGTC
AGACGTCCCGACAGTAACTCTAGCAAGACCACACCGAAGCTGTAGACGTCACTTTTGGCT
GTTATCCGACCAGTGGCAACGTATTCAGGTGCTGCATAGCCTTGAGTTCCCATCACTTGG
GTTGATACGTGTGTTCTGTCTCCGGGAAATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGTATGGTCCCATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATG
   
Sequence Name 31-K018601-022-275-D11C-8409
GenBank Accession LN499957 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K018601-022-275-D11C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATTTCCCGGAGACAGAACACACG
TATCAACCCAAGTGATGGGAACTCAAGGCTATGCAGCACCTGAATACGTTGCCACTGGTC
GGATAACAGCCAAAAGTGACGTCTACAGCTTCGGTGTGGTCTTGCTAGAGTTACTGTCGG
GACGTCTGACCGTAGACAAAACGAAAGTAGGAGTGGAACGAAATCTGGTTGATTGGGCAA
TACCTTACTTAGGAGACAAAAGGAAAGTGTTTAGGATAATGGACACAAAGCTTGGGGGAC
AATATCCTCACAAAGGAGCTTGTTTGACTGCTAATACTGCGTTGCAATGTCTTAATCAAG
AACCTAAGTTGAGGCCAAAGATGTCTGATGTGTTATCTACTCTTGAAGAGTTGGAGATGA
CGTTGAAGTCGGGTTCTATTTCAAACTCGGTTATGAAATTGACATCTTCTTCCTCTTCTT
TCACGGCGAAACAGAGAGTAAGAACTCCGGTTGCTGATCCGGTGTTGTCTAGTAGACGAT
GTCGACGTGTTCGGTGAAGTAGCGACAGAGATAGTTAGGTTTTATGAGTGCATGCATTTT
TTTTTGTTTTTTTTCCCTGGAAAAGGTGACATA

Last Updated on 06.12.2018 16:11