Sequences from confirmation amplicon


Confirmation sequences for line 275F04 and hit At1g69740 are shown.
Line ID 275F04
Locus At1g69740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26234697
Primers used for PCR 19sj - 8474, show primer details
   
Sequence Name 41-K116491-0022-275-F04C-19sj
GenBank Accession LN504219 [GenBank]
Sequencing primer gene specific primer, 19sj: 5'-TCAATGGAATCTACAAGAACCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K116491-0022-275-F04C-19sj
GCTCAATTTTGAGTCTAGGGACTCATCATAATCCATATTACAAAACACAAGATCACATAT
GCACGACACTAAAAGAATGGGATCAGAACAAGGCAAAATCACAATAGTCTCAAATGATTT
AAAACAGATATACTCAAACAGTTTAAAACACAGTCTTGTTGTCTATTCTACCGCTTCTCG
CCGCTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCACAATTATT
   
Sequence Name 09-K116490-0022-275-F04C-8409
GenBank Accession LN490353 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K116490-0022-275-F04C-8409
TCAGCGGCGANAGCGGTAGAATAGACACAAGACTGTGTTTTAAACTGTTTGAGTATATCT
GTTTTAAATCATTTGAGACTATTGTGATTTTGCCTTGTTCTGATCCCATTCTTTTAGTGT
CGTGCATATGTGATCTTGTGTTTTGTAATATGGATTATGATGAGTCCCTAGACTTCAAAA
TTTGGAGGCCATGATCTGAGTTTCTGAATGTGGTTCTTGTAANTTCCATTGAA

Last Updated on 06.12.2018 16:11