Sequences from confirmation amplicon


Confirmation sequences for line 276A01 and hit At3g02530 are shown.
Line ID 276A01
Locus At3g02530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:529945
Primers used for PCR 19gp - 8474, show primer details
   
Sequence Name 25-K112248-0022-276-A01C-19gp
GenBank Accession LN497511 [GenBank]
Sequencing primer gene specific primer, 19gp: 5'-TTTGATCTTTGTGGAGAAGAAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K112248-0022-276-A01C-19gp
TCTTACTGATCTAAACCATCACGGACAGCGTCCTTAATTTGAGCAATAGTGTGGTCATTA
GGCCCTATAAAGAAAACCAAATCATAATCATAGATAATCAATGACCACACATATGAAGAT
AAGCAAAATTGATCTTCAAAACTTAGCTGGCAAGAGATAGAAGTTTGGGCATTTGTACCT
TTTATGAGGATAGTACATGAATGAGGATTCTTCACTTGCTCAACAAATGTGTACTTCTCC
TCTCCAAGAACGTGCTCATATACAAGTCCAGCCCAACCAAGACAATCAGGGGTCAAGTCA
TCGACAGAGTTCACAGCTTCTCCACCACATGCCAAAACCAAACGTTCCATGTTCCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCC
   
Sequence Name 09-K112247-0022-276-A01C-8409
GenBank Accession LN490245 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K112247-0022-276-A01C-8409
GAGCATTTCATTGAATATATCCTGGAACTGGAACGTTTGGTTTTGGCATGTGGTGGAGAA
GCTGTGAACTCTGTCGATGACTTGACCCCTGATTGTCTTGGTTGGGCTGGACTTGTATAT
GAGCACGTTCTTGGAGAGGAGAAGTACACATTTGTTGAGCAAGTGAAGAATCCTCATTCA
TGTACTATCCTCATAAAAGGTACAAATGCCCAAACTTCTATCTCTTGCCAGCTAAGTTTT
GAAGATCAATTTTGCTTATCTTCATATGTGTGGTCATTGATTATCTATGATTATGATTTG
GTTTTCTTTATAGGGCCTAATGACCACACTATTGCTCAAATTAAGGACGCTGTCCGTGAT
GGTTTAAGATCAGTTAAGAATACCCTAGAAGATGAGTGTGTCGTATTAGTAAGTACCTTC
TTCTCCCAAGGGATCAA

Last Updated on 06.12.2018 16:11