Sequences from confirmation amplicon


Confirmation sequences for line 276C02 and hit At1g15230 are shown.
Line ID 276C02
Locus At1g15230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5243088
Primers used for PCR CQ18 - 8474, show primer details
   
Sequence Name 12-K105564-0022-276-C02C-CQ18
GenBank Accession LN491575 [GenBank]
Sequencing primer gene specific primer, CQ18: 5'-GACTCTCAGACAAATCCTTCAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K105564-0022-276-C02C-CQ18
TCTGATCCTTCTTCTCACTGCTTCTCTCAATACCTTCAACATCTAGATCCTTCAAACCAT
CAACGCAGCTCTTCCCTTCTTCTCCTTCGCCAGCCACCACCAAATCTCCGGCCTGTTTCG
ATTCAGCCAACTTCTCAATGCCTTTTCCAGCTAAACACGGCCCAGTATCCCTTTCATTGT
CTCCTCCAACGATAATCTTGTCTGCAGTTTCCGGAATAGGATCCGCTAAACAACCGTCTT
CCTCCTCGTGGTCGAATATCGACGCTGCGCGAGACTTCACGGCGTTAGCGGCTTTGCGAA
ATGCCAATATGGAGAAATCTACATATATGTATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 60-K105565w-0022-276-C02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K105565w-0022-276-C02C-8409
TTGGCATTTCGCNAGCCGCTAACGCCGTGAAGTCTCGCGCAGCGTCGATATTCGACCACG
AGGAGGAAGACGGTTGTTTAGCGGATCCTATTCCGGAAACTGCAGACAAGATTATCGTTG
GAGGAGACAATGAAAGGGATACTGGGCCGTGTTTAGCTGGAAAAGGCATTGAGAAGTTGG
CTGAATCGAAACAGGCCGGAGATTTGGTGGTGGCTGGCGAAGGAGAAGAAGGGAAGAGCT
GCGTTGATGGTTTGAAGGATCTAGATGTTGAAGGTATTGAGAGAAGCAGTGAGAAGAAGG
ATCAGAGTGATGAAGATGAAGAAGAGGAGATGAAACCGATCTTGGTTGAAGGATT

Last Updated on 06.12.2018 16:11