Sequences from confirmation amplicon


Confirmation sequences for line 276D06 and hit At2g03470 are shown.
Line ID 276D06
Locus At2g03470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1046884
Primers used for PCR KR41 - 8474, show primer details
   
Sequence Name 75-K100822-0022-276-D06C-8409
GenBank Accession LN513195 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K100822-0022-276-D06C-8409
CATTTACAATTGAATAGACAATCAGATCCTCTACTGCAACAAAGTCACGTCCAAAGTTAA
AACTTAGCAGAACAAAGTATATGAATTTAAACATTTAAAAGGGTTTCGGGTTGTTACCTA
AAACATTTGTCTTCTTCTCCATAGAAGCATGATGATATGGAATAGCTTCGTCAAATTGGT
TATGGTTTTTGTAACTAAGCTGTCTTGCATGCTTAGCATTGCACTTTTGCATCTCTTCAG
CATCATAAGGTCGCTTTAATCCCATTTATTTTAACAACCTGAATTGATTTAACAATCTAG
GGTATTAGTGTAATTTTTTAATTTTTAGTCAAAAAGTCAAACTTTAAACAGATATTAAAA
ATAAAAAAAAAACCCCAAATTCGAAAACCTAACCCTAGATTTTTCAATCCACGGCTAAAA
ACATGAAATCAAGAGTCCAAATTCAAACAATCGA
   
Sequence Name 75-K100834-0022-276-D06C-KR41
GenBank Accession LN513197 [GenBank]
Sequencing primer gene specific primer, KR41: 5'-TGCTGAAAGTTAATCGATTGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K100834-0022-276-D06C-KR41
ATTGAAAATCTAGGGTTAGGTTTTCGAATTTGGGGTTTTTTTTTTATTTTTAATATCTGT
TTAAAGTTTGACTTTTTGACTAAAAATTAAAAAATTACACTAATACCCTAGATTGTTAAA
TCAATTCAGGTTGTTAAAATAAATGGGATTAAAGCGACCTTATGATGCTGAAGAGATGCA
AAAGTGCAATGCTAAGCATGCAAGACAGCTTAGTTACAAAAACCATAACCAATTTGACGA
AGCTATTCCATATCATCATGCTTCTATGGAGAAGAAGACAAATGTTTTAGGTAACAACCC
GAAACCCTTTTAAATGTTTAAATTCATATACTTTGTTCTGCTAAGTTTTAACTTTGGACG
TGACTTTGTTGCAGTAGAGGATCTGATTGGTCTATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATTTCC

Last Updated on 06.12.2018 16:11