Sequences from confirmation amplicon


Confirmation sequences for line 276E09 and hit At5g59700 are shown.
Line ID 276E09
Locus At5g59700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24053587
Primers used for PCR 05yz - 8474, show primer details
   
Sequence Name 47-K123255-0022-276-E09C-05yz
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 05yz: 5'-CACCATTTCTCTAGTAAGTGTCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K123255-0022-276-E09C-05yz
CCAAAGAGTAAACATCTGATTTCTCTGTGAGCTGTTGTCTTCTGAAGTATTCTGGATCAA
GATATCCGAAACTTCCTTTGACCGCGGTACTCACATGAGTCTGATCGATCTCCGGTCCGG
TCTTAGACAGTCCAAAGTCTGCAACTTTAGCCATGAGATTCTCGTCAAGCAATATGTTGG
CGGATTTGACGTCTCTGTGAATCACGGGTTTCGCGTCACCGGTGTGGAGATAATGCAATC
CTCTTGCTGATCCAATGCATATCTCAAGACGTTGCTTCCAACTCAAGCTAAGAAGACCTG
AACCGTAAAGGTGACTCTTCAATGTTCCATTCTCCATATATTCGTAAACCAGAATCATCT
CGTTATTCTCGTCGCAGTATCCGATCAAAGAAACCAAATGGCGATGACGGAATTGTGACA
ACATTTCGATTTCTGTCCTGAACTCTGCAAGTCCTTGCTGAGACTTAGGATTCGCTCTTT
TAACAGCTACTTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAA
GTAGATGTCCGCACGGTTATT
   
Sequence Name 15-K123254-0022-276-E09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K123254-0022-276-E09C-8409
TCGGACTGAGCCATTTCAAAGTAGCTGTTAAAGAGCGAATCCTAAGTCTCAGCAAGGACT
TGCAGAGTTCAGGACAGAAATCGAAATGTTGTCACAATTCCGTCATCGCCATTTGGTTTC
TTTGATCGGATACTGCGACGAGAATAACGAGATGATTCTGGTTTACGAATATATGGAGAA
TGGAACATTGAAGAGTCACCTTTACGGTTCAGGTCTTCTTAGCTTGAGTTGGAAGCAACG
TCTTGAGATATGCATTGGATCAGCAAGAGGATTGCATTATCTCCACACCGGTGACGCGAA
ACCCGTGATTCACAGAGACGTCAAATCCGCCAACATATTGCTTGACGAGAATCTCATGGC
TAAAGTTGCAGACTTTGGACTGTCTAAGACCGGACCGGAGATCGATCAGACTCATGTGAG
TACCGCGGTCAAAGGAAGTTTCGGATATCTTGATCCAGAATACTTCAGAAGACAACAGCT
CACAGAGAAATCAGATGTTTACTCTTTTGGAGTGGTTATGTTCGAGGTTCTCTGCGCGAG
ACCTGTTATAGACCCGACACTTACTAGAGAAATGGTG

Last Updated on 06.12.2018 16:11