Sequences from confirmation amplicon


Confirmation sequences for line 277E05 and hit At3g57470 are shown.
Line ID 277E05
Locus At3g57470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21270086
Primers used for PCR 04ay - 8474, show primer details
   
Sequence Name 19-K107199-0022-277-E05C-04ay
GenBank Accession LN494783 [GenBank]
Sequencing primer gene specific primer, 04ay: 5'-CCCCAACATTGAGGTTCTTAAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K107199-0022-277-E05C-04ay
GAGTGGAAAGGAGAGTTTTGTCTATACCTCTGCATCTATGCGGTTGAATTTGAGTGTCCC
GGTTTGAATCTCTGCCCAGTAAAACCAGGATTCCTCATCCAAGTTCTTGTCCTTTTCAAG
CTTCATATCTATCAAATTTGTTACATTGCTCTGAGGAGAAAAGTCATGTCCGATAGTACA
TCAATGTTTATCTCAAGCAAATCAAATATCATCTAGCTTGTGTCTCTAACCTTGAATTCT
TCATCGCTCATATTGTAAAATTTACTCTCCAGGTCCTTTAGTAGTGACTCAACTCTCGAA
TCAATATGTCCTGGACCCTATTAACGATAAACTCAAAACCAATTAAAGAGATCTGCATTG
ATTATGCAAAGTCTTGAGTGGCTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTA
   
Sequence Name 03-K107198-0022-277-E05C-8409
GenBank Accession LN487509 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K107198-0022-277-E05C-8409
GAGCCATTTACAATTGAATTATCCTGGAGCCACTCAAGACTTTGCATAATCAATGCAGAT
CTCTTTAATTGGTTTTGAGTTTATCGTTAATAGGGTCCAGGACATATTGATTCGAGAGTT
GAGTCACTACTAAAGGACCTGGAGAGTAAATTTTACAATATGAGCGATGAAGAATTCAAG
GTTAGAGACACAAGCTAGATGATATTTGATTTGCTTGAGATAAACATTGATGTACTATCG
GACATGACTTTTCTCCTCAGAGCAATGTAACAAATTTGATAGATATGAAGCTTGAAAAGG
ACAAGAACTTGGATGAGGAATCCTGGTTTTACTGGGCAGAGATTCAAACCGGGACACTCA
AATTCAACCGCATAGATGCAGAGGTAATAGACAAAACTCTTCCTTTTTCCAACTTCTTTC
TAGTAATCTCCTATTTAAGAACCTCAATGTTGGGG

Last Updated on 06.12.2018 16:11