Sequences from confirmation amplicon


Confirmation sequences for line 277G01 and hit At3g46330 are shown.
Line ID 277G01
Locus At3g46330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17022829
Primers used for PCR PP21 - 8474, show primer details
   
Sequence Name 48-K103008-0022-277-G01C-8409
GenBank Accession LN506476 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K103008-0022-277-G01C-8409
TCGGGCACTATTCCCTCAAACTGTTATTTGACAAATCCCTGGGATGTGAAAAACAATGAA
CCCATATATGCTTTGTAATTATATATCAAATATCTGTAGAAAAGAAAAAAATAAACATGC
TGCTTTAATTAATCACGTACTAACAATACTTACAAACTTTCTAGATGCGCCAGATTCTGA
AAGTTAGACACTATAGTCCCACTCAAGCCACTCGATGATAAATTTCTGGTTATAATAAAC
AAATTATAAGTTTAGATTATATATAAGCATGATAATTTAGTAATTTTAAAAGTGTGTGTG
ACATAGTTAAATCTTACAAAGAAATTATTCTTGGTGGTGCAGATATATCTGTGATGTTAC
AATTCAATCCATCCCACAAGAATTGTTTCGGTACGCATGGATCTCCTTGCCAACTGATTC
TACTCAGTCCATAAGTAGTTCTAATGTTTTTGATAGCAACCACTAGCATAAAATTTGATC
GATGCGAAAAAAATATTTAATGAATTAGTTTTCATCGATATGGAAGAACTTATAACAAAA
CAAAATGATAAAATAACTGTCTCTTTTAGTTTGATACCTTCAATTTCATTTGTCTGAGAT
TGTGGAAG
   
Sequence Name 96-K103009-0022-277-G01C-PP21
GenBank Accession LN515388 [GenBank]
Sequencing primer gene specific primer, PP21: 5'-CTTCCACAATCTCAGACAAATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>96-K103009-0022-277-G01C-PP21
TTATTTTATCATTTTGTTTTGTTATAAGTTCTTCCATATCGATGAAAACCATTTCATTAC
ATATTTTTTTCGCATCGATCAAATTTTATGCTAGTGGTTGCTATCAAAAACATTAGAACT
ACTTATGGACTGAGTAGAATCAGTTGGCAAGGAGATCCATGCGTACCGAAACAATTCTTG
TGGGATGGATTGAATTGTAACATCACAGATATATCTGCACCACCAAGAATAATTTCTTTG
TAAGATTTAACTATGTCACACACACTTTTAAAATTACTAAATTATCATGCTTATATATAA
TCTAAACTTATAATTTGTTTATTATAACCAGAAATTTATCATCGAGTGGCTTGAGTGGGA
CTATAGTGTCTAACTTTCAGAATCTGGCGCATCTAGAAAGTTTGTAAGTATTGTTAGTAC
GTGATTAATTAAAGCAGCATGTTTATTTTTTTCTTTTCTACAGATATTTGATATATAATT
ACAAAGCATATATGGGTTCATTGTTTTTCACATCCCAGGGATTTGTCAAATAACAGTTTG
AGTGGAATAGTGCCCGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTCACGCCCAAATGG

Last Updated on 06.12.2018 16:11