Sequences from confirmation amplicon


Confirmation sequences for line 279H03 and hit At4g10610 are shown.
Line ID 279H03
Locus At4g10610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6558459
Primers used for PCR JZ10 - 8474, show primer details
   
Sequence Name 36-K103233-0022-279-H03C-8409
GenBank Accession LN502187 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K103233-0022-279-H03C-8409
GAGCCTTGCCATTGAATATACTGGTTTTTTCTTGTGTTTCATCACATTCTGACTGCAAAG
TTATGGTTTTTTAGACTCAAGAGGTTTGATTTTAGTCCTGTCTTTGTTCTTAGACTGAAG
ATGAGCGTGAGATGTGTGCAAGAACTATCTACTGCACTAACATTGACAAGAAGGTGACTC
TCACTCGAATAAATTGGTTTCCTTTGCCAAACTATTCTGAAACTTAATGTTGCTAACTTT
GCTTAGTTTCACTTTTCAACAGCTCACTCAAACCGACATTAAACTCTTCTTTGAGTCTGT
GTGTGGCGAGGTGTACCGTCTGAGGCTACTGGGCGATTATCATCATCCAACTCGCATCGG
CTTCGTGGAGTTTGTCATGGTAAGCAAATACTTCTGTTATCTCTCTTTCTTCCAACCTTC
CATTTGTCAACTATAACGCTNATGCATTTTCATTAGTTAAGTATGATCTTTTCACTCTTC
CGCAGGCTGAAAGCGCGATAGCGGCTCTCAACTGCAGTGGTGTCCTTCTGGGTTCTTT
   
Sequence Name 84-K103234-0022-279-H03C-JZ10
GenBank Accession LN514418 [GenBank]
Sequencing primer gene specific primer, JZ10: 5'-TAAAGAACCAAGAAGGACACCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K103234-0022-279-H03C-JZ10
CTTCGCCTGCGGAAAGTGAAAGATCATACTTAACTAATGAAAATGCATAAGCGTTATAGT
TGACAAATGGAAGGTTGGAAGAAAGAGAGATAACAGAAGTATTTGCTTACCATGACAAAC
TCCACGAAGCCGATGCGAGTTGGATGATGATAATCGCCCAGTAGCCTCAGACGGTACACC
TCGCCACACACAGACTCAAAGAAGAGTTTAATGTCGGTTTGAGTGAGCTGTTGAAAAGTG
AAACTAAGCAAAGTTAGCAACATTAAGTTTCAGAATAGTTTGGCAAAGGAAACCAATTTA
TTCGAGTGAGAGTCACCTTCTTGTCAATGTTAGTGCAGTAGATAGTTCTTGCACACATCT
CACGCTCATCTTCAGTCTAAGAACAAAGACAGGACTAAAATCAAACCTCTTGAGTCTAAA
AAACCATAACTTTGCAGTCAGAATGTGATGAAACACAAGAAAAAACCATGTATATTCAAT
TGGCAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACNAATTACGATCCGTCGTATTT
ATNGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTAC
TT
Primers used for PCR JZ09 - 8474, show primer details
   
Sequence Name 43-K103233-0022-279-H03C-OriL
GenBank Accession LN504801 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K103233-0022-279-H03C-OriL
TTCGGAAGAAGCCATTTACTGTAGATTTCGTGGCAACCTTGGCAAGAAGGTTGGGTTAAC
CGGTGCAATAGCTGTCTTAGACGGCATAACCTTCACAGGATAGAATCCAAGCATCGTCCC
AGACAAATTCAGAGCAGTCCTTGCACCCACTACAAGTCCCAAAATGACAAACCATAAACC
AAGCACTTACAGGCATTATCCAATAAACAGATTACATAGAGAAACTAATGATACCTTCAT
CAGTGAACTCGATGAAAGCAAACCTAAGTACTGAGTTGGGATCACCACATATACGACAGT
CAACAACCTAAACAACCAAATACAGTCAACACCATAACACAACACAAACAAAGATGAACT
TTAAGAAGTTGCTTAAACCTGTCCAAAGCCGATAAACAAACCAGCAAGCTGCTCCTCAGT
GACCTTAAACCCAAGAATAAGATTACTAATAAGTGATTATACACTAAAGGCTGAGAATGA
ACAATTGAAGAAAGGTGGAAACTTTTTAAAACCTGTTGGTCAATATCAGACACATAGACN
GTTCTTCTTATGATCTCTTCACGCTGAGCCAAACTTGTCCTAGGATTCATTCTCCGTTTC
CCTTGTTGTCCGAACGACTTCTTCTGTTTCAAATCAAATCATTTTGACACATAAAGGTGA
GATTTTTAAAACTCACAAACACACACAAGAAGAAACAGAGAAACCAAAATGAAGAATCAT
CAAAAACCCTAAAGAACAATTTGATTCANAACCCCAAAATCACAGAGAAGAGAGAAGAGA
GATGTTCTTACCCGACGAAAGCTACCGTCTTCGTTGACGGGGAAATTTCCGGCAGCAACA
AAGCCATTATTAACGGCGAAGAAACCGCCGTTAAA
   
Sequence Name 91-K103234-0022-279-H03C-JZ09
GenBank Accession LN515115 [GenBank]
Sequencing primer gene specific primer, JZ09: 5'-AGCCAGTTGTTAATGGTTTTAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K103234-0022-279-H03C-JZ09
TGGCTTTGTGCTGCCGGAATTTCCCCGTCACGAAGACGGTAGCTTTCGTCGGGTAAGAAC
ATCTCTCTTCTCTCTTCTCTGTGATTTTGGGGTTTTGAATCAAATTGTTCTTTAGGGTTT
TTGATGATTCTTCATTTTGGTTTCTCTGTTTCTTCTTGTGTGTGTTTGTGAGTTTTAAAA
ATCTCACCTTTATGTGTCAAAATGATTTGATTTGAAACAGAAGAAGTCGTTCGGACAACA
AGGGAAACGGAGAATGAATCCTAGGACAAGTTTGGCTCAGCGTGAAGAGATCATAAGAAG
AACTGTCTATGTGTCTGATATTGACCAACAGGTTTTAAAAAGTTTCCACCTTTCTTCAAT
TGTTCATTCTCAGCCTTTAGTGTATAATCACTTATTAGTAATCTTATTCTTGGGTTTAAG
GTCACTGAGGAGCAGCTTGCTGGTTTGTTTATCGGCTTTGGACAGGTTTAAGCAACTTCT
TAAAGTTCATCTTTGTTTGTGTTGTGTTATGGTGTTGACTGTATTTGGTTGTTTAGGTTG
TTGACTGTCGTATATGTGGTGATCCCAACTCAGTACTTAGGTTTGCTTTCATCGAGTTCA
CTGATGAAGGTATCATTAGTTTCTCTATGTAATCTGTTTATTGGATAATGCCTGTAAGTG
CTTGGTTTATGGTTTGTCATTTTGGGACTTGTAGTGGGTGCAAGGACTGCTCTGAATTTG
TCTGGGACGATGCTTGGATTCTATCCTGTGAAGGTTATGCCGTCTAATACAGCTATTGCA
CCGGTTAACCCAACCTTCTTGCCAAGGTTGCCACGAAATCTACATGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTAAGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTC

Last Updated on 06.12.2018 16:11