Sequences from confirmation amplicon


Confirmation sequences for line 281A03 and hit At2g31100 are shown.
Line ID 281A03
Locus At2g31100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13258081
Primers used for PCR DE55 - 8474, show primer details
   
Sequence Name 53-K104987-0022-281-A03C-DE55
GenBank Accession LN508148 [GenBank]
Sequencing primer gene specific primer, DE55: 5'-GTGGATGTATAAAGGGATAGCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K104987-0022-281-A03C-DE55
GGTTACTCCGGGAAAACATAACAGCTGATTCAAGTGGGAAATCGAAATCGTTAGCCCACT
CGTACAGCTGAATGGTCCCTCGCCATGCAACAACAATGCCCCTCCTCCCTAACAACTTCT
TGCCTTCGTCTGTAGCCACCGCAATGTAACCCAACTAGTTAGACTCTTTGTTCCATGCTT
CCCTTGACAATGATTTGATTATGAAACATTCTGGTAACCTTATAGACGAAGTTCCATATA
TGTACTTGGTCACCTCGTACCTGGATGACAACATTTTTTCACACGTTTCATATATGAGTA
GGCATGAAAACTAAACAAAAATTTCTAGAACCGAAACGATATGGATGATACTAGTGGATA
CGTTTGTTACCTGAAAGGGTTGGCTTTGAGGTAACCGGTTCGAGCAAAGAGCTCTTCTTT
GGTGTAGCAGCTATCTCCTACATATTTAGACCGGCGGTCACTATTAAAGGCAAGGTACCC
TACCTCAGCCATATCACCATAGTGTAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 37-K104988-0022-281-A03C-8409
GenBank Accession LN502608 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K104988-0022-281-A03C-8409
TTTACAATTGAATATATCCTACACTATGGTGATATGGCTGAGGTAGGGTACCTTGCCTTT
AATAGTGACCGCCGGTCTAAATATGTAGGAGATAGCTGCTACACCAAAGAAGAGCTCTTT
GCTCGAACCGGTTACCTCAAAGCCAACCCTTTCAGGTAACAAACGTATCCACTAGTATCA
TCCATATCGTTTCGGTTCTAGAAATTTTTGTTTAGTTTTCATGCCTACTCATATATGAAA
CGTGTGAAAAAATGTTGTCATCCAGGTACGAGGTGACCAAGTACATATATGGAACTTCGT
CTATAAGGTTACCAGAATGTTTCATAATCAAATCATTGTCAAGGGAAGCATGGAACAAAG
AGTCTAACTAGTTGGGTTACATTGCGGTGGCTACAGACGAAGGCAAGAAGTTGTTAGGGA
GGAGGGGCATTGTTGTTGCATGGCGAGGGACCATTCAGCTGTACGAGTGGGCTAACGATT
TCGATTTCCCACTTGAATCAGCTGTTATGGTTTTCCCTGGAGCTAACCCGAATGACGAGC
CTCGCGTAGCAAATGGTTGGCTATCC

Last Updated on 06.12.2018 16:11