Sequences from confirmation amplicon


Confirmation sequences for line 283B06 and hit At1g02120 are shown.
Line ID 283B06
Locus At1g02120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:397402
Primers used for PCR KP39 - 8474, show primer details
   
Sequence Name 33-K030468-022-283-B06C-8409
GenBank Accession LN500876 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K030468-022-283-B06C-8409
ATGAAGCCATTACAATTGAATATATCCTGATCAATTGAATCTATAAATTGTTTGAAATTT
AAATGGTCAATGGGAAACGGTCTCTTAATGGTTTTGCTAAATAAGGCCTATCAGGATGGG
GTAGCTTAGCTTTATGATCTTGTCTTTTGGTGCTACTTCACAAGTTGCACTAGTTCTCAG
TTTGTCCCAACGAGCTTGCACTCAGTATGGAGATCCTATATTACTGAACCGGTTATTTCG
TTTTTGCATACATGATCTCTCATCTATTTCTTTTGGTTATCGTGATCTCTTTTCTTGTTC
TCATTTGTATTCACCACACAAATTTGTGTTCCTTGCACGCAGTGTCATCTGACTTTACAA
AGGTAGCAGAGGCAAAGTTTTCGGTAATGTCTCGTTCTTTGTCTTATCAGCTAGAGGAAA
TTATGGAACCATTCTCATTCAAATTTATGAGAATATAAGCATTGACTGGATGCAGAAGTT
TCATATACCTAAATAGACTTTAGTTTATATTTGCAGATTCCAGTAGAAGAGTTCTTTAGA
TTGTTTTTTTCAGATGGTGCTGTCAGTTTTGTTGAATCTNTCCATAAAAATTGTGGAGAC
AAAGGTAAAGCTATAAGTGATGGAATCATTTTGC
   
Sequence Name 34-K030468-022-283-B06C-KP39
GenBank Accession LN501295 [GenBank]
Sequencing primer gene specific primer, KP39: 5'-AGCTAAACACAGGAGCAAAATGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K030468-022-283-B06C-KP39
CAAACAAAAACTGAACAGCACCATTCTGAAAAAAAACAATCTAAAGAAACTCTTTCTACC
GGAAATCTGCAAATATAAACTAAAGTCTATTTAGGTATATCGAAACTTCTGCATCCAGTC
AACGCTTATATTCTCATAAATTTGAATCGAGAATCGGTTCCATAATTTCCTCTAGCTGAT
AAGACAAAGAACGAGACATTACCGAAAACTTTGCCTCTGCTACCTTTGTAAAGTCAGATG
ACACTGCGTGCAAGGAACACAAATTTGTGTGGTGAATACAAATGAGAACAAGAAAAGAGA
TCACGATAACCAAAAGAAATAGATGAGAGATCATGTATGCAAAAACGAAATAACCGGTTC
AGTAATATAGGATCTCCATACTGAGTGCAAGCTCGTTGGGACAAACTGAGAACTAGTGCA
ACTTGTGAAGTAGCACCAAAAGACAAGATCATAAAGCTAAGCTACCCCATCCTGATAGGC
CTTATTTAGCAAAACCATTAAGAGACCGTTTCCCATTGACCATTTAAATTTCAAACAATT
TATAGATTCAATTGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTTCATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTT

Last Updated on 06.12.2018 16:11