Sequences from confirmation amplicon


Confirmation sequences for line 283D01 and hit At1g19020 are shown.
Line ID 283D01
Locus At1g19020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6568290
Primers used for PCR LB21 - 8474, show primer details
   
Sequence Name 42-K033737-022-283-D01C-8409
GenBank Accession LN504370 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K033737-022-283-D01C-8409
ATTTACATTGAAGCCATTTTTGAACCCTTGTCCCATTTTCTCCCTGTACTTGGTCGTGTT
TGACGTTGCTCCACTTCCGCTGCTTCTCACGACGGCGCCGCTGCCGATTCGGCCTCCTTT
GGCAGAGTCGCCGCTATTGTCCCACTGGTCAGCCCACGATGTTCCGTTCATTATTGTATA
TCGTCTTCTCAAAAGTTTTAAAATCGAAATGGAATTTGTACTTTGAAGTTTATTTGTGAT
GTTCTCTCGGTTCCTCTGTTTTGTTTATATACACATTCATATTTCAAGAGACGCGTTATG
TATGGTCAAACCTTGTGTTCGTGAACAAATATGACTTCTATTGATTTGCTTCTTCTTTAA
CTTTATTTTATATGAATTGTCACACTCAATATAATCTTCTGGAACATTAAAAGAATCTCT
CGTGGAAAAAATTGAAGTAAATTCTTTATATTTTATAGAATCAAGTACACATTGAGGAAA
CTTCTTCAAATTCCCCGAGTCTAGACGAAGACTCTTCAATGTTTTGACTGNACGACTCA
   
Sequence Name 44-K033737-022-283-D01C-LB21
GenBank Accession LN505091 [GenBank]
Sequencing primer gene specific primer, LB21: 5'-GAGTCGTCAAGTCAAAACATTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K033737-022-283-D01C-LB21
GGATTTGAGAGTTTCCTCAATGTGTACTTGATTCTATAAAATATAAAGAATTTACTTCAA
TTTTTTCCACGAGAGATTCTTTTAATGTTCCAGAAGATTATATTGAGTGTGACAATTCAT
ATAAAATAAAGTTAAAGAAGAAGCAAATCAATAGAAGTCATATTTGTTCACGAACACAAG
GTTTGACCATACATAACGCGTCTCTTGAAATATGAATGTGTATATAAACAAAACAGAGGA
ACCGAGAGAACATCACAAATAAACTTCAAAGTACAAATTCCATTTCGATTTTAAAACTTT
TGAGAAGACGATATACAATAATGAACGGAACATCGTGGGCTGACCAGTGGGACAATAGCG
GCGACTCTGCCAAAGGAGGCCGAATCGGCAGCGGCGCCGTCGTGAGAAGCAGCGGAAGTG
GAGCAACGTCAAACACGACCAAGTACAAGGAGAAAATGGGACAAGGGTTCAAAAATGGCT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACATTC
Primers used for PCR LB22 - 8474, show primer details
   
Sequence Name 45-K033737-022-283-D01C-LB22
GenBank Accession LN505438 [GenBank]
Sequencing primer gene specific primer, LB22: 5'-GTAAACCTCTTGGCATCTCTATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K033737-022-283-D01C-LB22
GATATAACCATGACTAGGGTTTCTGCTTCCGCCGATAATTTTAGAGTGAAATGTGATTCT
GGTTGCTTTCTAGGGTTTTGGGTTCTCCGATTGATTTACTCTGAAGAAGAACCACTTCAG
ATCCGGCGGATAAAAATTGTTTCTGCAGAAGCGAGGGAAAATTACCCTCCGATCTTAAAA
GTAAAATTTATAAAATTACACGTTATATACTTCGAAATAAATCGTTTCTTATTTTTATAT
ATTTATGATTTAATCGCATAATATGATTTTGTCTAAACGATAATTCGTTGTTATTATAGA
TGTAATAATTTAAAAATTAAAGAGAAAAACAATTTAATTAATCCATGAACCCACCCAAAC
TCGAACAATTTACCACAGTAAACAAAGCATTCAAATCAAACAAGCTTAGAAAAATATAGA
TCAGACATAATACAGTATTTGGCAAGTGAATAAATTACTAATACTCATATACAAAGGAGT
TTACAGAAATCTTTATTGTTTGTGTGTGGTTTTGTGATACTTGTCCTTGACCCAACGAAA
ACCAATAGCAGAACCAGTCTTGAGCTTCTTAAAACCAGAAGAAGCTACAGCTTTGGATAT
ATATTCAATTGCTGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAGATCTTTATNTCGACGTGTCTACATC

Last Updated on 06.12.2018 16:11