Sequences from confirmation amplicon


Confirmation sequences for line 283D10 and hit At1g78490 are shown.
Line ID 283D10
Locus At1g78490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29530144
Primers used for PCR CQ29 - 8474, show primer details
   
Sequence Name 12-K105632-0022-283-D10C-CQ29
GenBank Accession LN491576 [GenBank]
Sequencing primer gene specific primer, CQ29: 5'-AACATGACTTTCACCAACATGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K105632-0022-283-D10C-CQ29
ATACATATTTGTATGAGATATTTACAGTGGAAGATATGACATTAATCTATAGGTTAGCAA
TGAGGTGCTTCGCCTAGCAAACACGACCCCTTTGTTGTTTCGAAAGGCGGTGCAAGATGT
TGAGATCAAAGGCAAGTACTACTTGGAAGTTATACATGTATGTATAAAAATATTCTCTGA
ATCACTAGAAGAAACTTTAATCCTGGGCAGGATATACAATTCCGGCTGGTTGGATTGTGG
CGGTTGCACCTTCAGCGGTTCATTTTGATCCTGCAATCTATGAGAACCCATTTGAGTTTA
ATCCATGGAGATGGGAGGTCTTAATATAATTTTCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 28-K105633-0022-283-D10C-8409
GenBank Accession LN498846 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K105633-0022-283-D10C-8409
TCGGACTGAGCCATTTACATTGAATATATCCTGAAAATTATATTAAGACCTCCCATCTCC
ATGGATTAAACTCAAATGGGTTCTCATAGATTGCAGGATCAAAATGAACCGCTGAAGGTG
CAACCGCCACAATCCAACCAGCCGGAATTGTATATCCTGCCCAGGATTAAAGTTTCTTCT
AGTGATTCAGAGAATATTTTTATACATACATGTATAACTTCCAAGTAGTACTTGCCTTTG
ATCTCAACATCTTGCACCGCCTTTCGAAACAACAAAGGGGTCGTGTTTGCTAGGCGAAGC
ACCTCATTGCTAACCTATAGATTAATGTCATATCTTCCACTTGTAAATATCTCATACAAA
TATGTATATATATAGATGGGGGTATTTAATCTACCATGTTGGTGAAAGTCATGT
Primers used for PCR 18ah - 8474, show primer details
   
Sequence Name 41-K118837-0022-283-D10C-18ah
GenBank Accession LN504266 [GenBank]
Sequencing primer gene specific primer, 18ah: 5'-ACAGCCGCAAAGAGTACATAGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K118837-0022-283-D10C-18ah
ACAAAGTTTAACTCATTTGACAAGATAGATACTTTTTAAAAATTATAGAGATCGCATATT
ACATATAAAAAGACTCCAGAGATTTTGCGGAAGCAGGAAATGAGATTATTGCATATAATG
ATCTGATTATTAAGATTTGGAAACTGCATGCATGGTTCACTTGGTAGGAGACTGAGAGAT
GTTTATAAGCAGATCCTTGGTGTATTGATGGAATGGTGAACGAATGATCTCGGAGTCTTG
GACCATTGAGAAATCGTAATATGCCACAAGATGATGGAGGAAGATTGCCATTTGAAGCCG
TGAAAACTCTGCACCTACACAAAGTCTAACTCCATATCCAAACGCCATGAACGTTTTAGA
TCCCCAAATCATTTCTTTCCCCTGCCACAACCAACCAAATCCCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAATTATTA
   
Sequence Name 17-K118836-0022-283-D10C-8409
GenBank Accession LN494077 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118836-0022-283-D10C-8409
TTGGGATTTGGTTGGTTGTGGCAGGGGAAAGAAATGATTTGGGGATCTAAAACGTTCATG
GCGTTTGGATATGGAGTTAGACTTTGTGTAGGTGCAGAGTTTTCACGGCTTCAAATGGCA
ATCTTCCTCCATCATCTTGTGGCATATTACGATTTCTCAATGGTCCAAGACTCCGAGATC
ATTCGTTCACCATTCCATCAATACACCAAGGATCTGCTTATAAACATCTCTCAGTCTCCT
ACCAAGTGAACCATGCATGCAGTTTCCAAATCTTAATAATCAGATCATTATATGCAATAA
TCTCATTTCCTGCTTCCGCAAAATCTCTGGAGTCTTTTTATATGTAATATGCGATCTCTA
TAATTTTTAAAAAGTATCTATCTTGTCAAATGAGTTAAACTTTGTTTATATATGTCTTGA
ATCTGATCACATGCATGGGTTCCTAATTCTATGTACTCTTTGACGGCTGT

Last Updated on 06.12.2018 16:11