Sequences from confirmation amplicon


Confirmation sequences for line 283E02 and hit At5g03270 are shown.
Line ID 283E02
Locus At5g03270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:783252
Primers used for PCR FV95 - 8474, show primer details
   
Sequence Name 02-K100894-0022-283-E02C-8409
GenBank Accession LN486953 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K100894-0022-283-E02C-8409
CATATTACGATCTTTTAATGTCTGCTCAGAAATATAAATCTTACAGATATAAATTGCGAA
CATAAATTTTTTCTTAAATTGGATAAGAAACAGATGTCCTTTGTCGACATCTATTTTCAC
TGCTTTTGGATGTTGATGGATCAAGGACTTTTTCTGATTATTCACTTTAATTATTTCAAA
ACTATTTGTCGTGATTCTTTTAAATTCTTTGACTCCTGGAATCTCCCACATATCATATTA
ACCACATGTTATTACTTATTAAACGCACTCTTTAAAGCTCTCTATAATGACTAATAAAAA
GGTTTTTATAAAAAAGCACTAACATTACATTATGTAGGGGAAAAAACAAACACTAAAACA
TCACCATACATATTTGCATC
   
Sequence Name 02-K100902-0022-283-E02C-FV95
GenBank Accession LN486954 [GenBank]
Sequencing primer gene specific primer, FV95: 5'-GAGTTCCCCAATGTATATGATGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K100902-0022-283-E02C-FV95
GTGATGTTTAGTGTTTGTTTTTTCCCCTACATAATGTAATGTTAGTGCTTTTTTATAAAA
ACCTTTTTATTAGTCATTATAGAGAGCTTTAAAGAGTGCGTTTAATAAGTAATAACATGT
GGTTAATATGATATGTGGGAGATTCCAGGAGTCAAAGAATTTAAAAGAATCACGACNAAT
AGTTTTGAAATAATTAAAGTGAATAATCAGAANAAGTCCTTGATCCATCAACATCCAAAA
GCAGTGAAAATAGATGTCGANCAAGGACATCTGTTTCTTATCCCATTTAAGAAAAAATTT
ATGTTCGCAATTTATATCTGTAAGATTTATATTTCTGAGCAGACATTAAAAGATCGTAAT
ATGATGTAAATGGCTTCATGTCCCGGAAAT

Last Updated on 06.12.2018 16:11