Sequences from confirmation amplicon


Confirmation sequences for line 284C12 and hit At2g24850 are shown.
Line ID 284C12
Locus At2g24850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10583558
Primers used for PCR BM57 - 8474, show primer details
   
Sequence Name 11-K104415-0022-284-C12C-BM57
GenBank Accession LN491098 [GenBank]
Sequencing primer gene specific primer, BM57: 5'-TACACCTACGACCATCTCAACAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K104415-0022-284-C12C-BM57
TTGCTTTGATGTAATATAAGTTTGACATTTAATGTAATATAAGTTTTTTTGAAGTGTAAA
ACAACTAACGTTTTTTGGTAATATATCGACTAATCTTTAGGTCGCGGAGATGGCTAGAAA
ACTCGGTATAATGATAATATCCGACGAAGTATATGATCATGTTGTATATGGAGACAAGCC
TTTTATTCCCATGGGGAAGTTTGCATCAATAGCTCCGGTGATCACGCTCGGATCCATATC
CAAAGGATGGGTCAACCCAGGCTGGAGAGTTGGCTGGATCGCCATGAACGATCCTAATGG
TATCTTTGTATCTACAGGGGTCAGTCAACTTCTAAACACTATAGTTTTACTTAAAATAAG
ATTACCGCACCAATTAAGAAGATTCTAACTAATATATGGGCCTTTCGTTCACCAACAGGT
AGTTCAAGCAATAGAGGATTTTCTTGATTTAACTCCACAGCCTTCATTTATTCTCCAGGT
TTGTAATCTCTCACTATCATCGTAAGCTATAAGTTTTGGTTCTCTAAATAATGTGAGATT
ATTATGGATTTTGATAGGAAGCACTTCCTGATATATTGGAGAAAACACCTAAAGAGTTCT
TCGAGAAGAAGATCAAAGCCATGAGACGCAACGTCGAGCTTTCATGTGAGAGGCTCAAGG
ATATTCCTTGTCTCTTTTGTCCCAAGAAACCCGAATCTTGTTCTTATTTATGGATTCTTT
TTCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCNAAATGTAG
   
Sequence Name 27-K104416-0022-284-C12C-8409
GenBank Accession LN498369 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K104416-0022-284-C12C-8409
TTGAAGAAAAAGAATCCATAAATAAGAACAAGATTCGGGTTTCTTGGGACAAAAGAGACA
AGGAATATCCTTGAGCCTCTCACATGAAAGCTCGACGTTGCGTCTCATGGCTTTGATCTT
CTTCTCGAAGAACTCTTTAGGTGTTTTCTCCAATATATCAGGAAGTGCTTCCTATCAAAA
TCCATAATAATCTCACATTATTTAGAGAACCAAAACTTATAGCTTACGATGATAGTGAGA
GATTACAAACCTGGAGAATAAATGAAGGCTGTGGAGTTAAATCAAGAAAATCCTCTATTG
CTTGAACTACCTGTTGGTGAACGAAAGGCCCATATATTAGTTAGAATCTTCTTAATTGGT
GCGGTAATCTTATTTTAAGTAAAACTATAGTGTTTAGAAGTTGACTGACCCCTGTAGATA
CAAAGATACCATTAGGATCGTTCATGGCGATCCAGCCAACTCTCCAGCCTGGGTTGACCC
ATCCTTTGGATATGGATCCGAGCGTGATCACCGGAGCTATTGATGCAAACTTCCCCATGG
GAATAAAAGGCTTGTCTCCATATACAACATGATCATATACTTCGTCGGATATTATCATTA
TACCGAGTTTTCTAGCCATCTCCGCGACCTAAAGATTAGTCGATATATTACCAAAAAACG
TTAGTTGTTTTACACTTCAAAAAAACTTATATTACATTAAATGTCAAACTTATTATATAC
ATCAAAAGCTAAATGGAGAAAAACAAATATACCTTGTTG
Primers used for PCR BM58 - 8474, show primer details
   
Sequence Name 12-K104415-0022-284-C12C-BM58
GenBank Accession LN491563 [GenBank]
Sequencing primer gene specific primer, BM58: 5'-TATCGATATCCTCACCCAATTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K104415-0022-284-C12C-BM58
TTTCACACAAAATAAATATAAATAAGTAAATAAAAAAAGATTGATCATAATTAACGATGC
AAATTTATAAATTAAAATCATATGTAAGAAGATGACTATGGGAGCCCCAAGTTCTTAAGA
TGTTGTATATTGTTAAAGAGATCAATACCTGGTATAAGGATAAGACTCTCCTCACTAACT
AGCTTCGTGCAGAAATCAAAATCATTTTTGATATTATTCAACATTGATGTGTCAAGCTTC
AACTGTAAAATAAATCGTTTAAGCTAATCATTTATTATTATAAGCAAAATATGGAGCATC
CAATAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCA
   
Sequence Name 28-K104416-0022-284-C12C-OriL
GenBank Accession LN498830 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K104416-0022-284-C12C-OriL
GCCATTTACATTGAATATATCCTGCTATTGGATGCTCCATATTTTGCTTATAATAATAAA
TGATTAGCTTAAACGATTTATTTTACAGTTGAAGCTTGACACATCAATGTTGAATAATAT
CAAAAATGATTTTGATTTCTGCACGAAGCTAGTTAGTGAGGAGAGTCTTATCCTTATACC
AGGTATTGATCTCTTTAACAATATACAACATCTTAAGAACTTGGGGCTCCCATAGTCATC
TTCTTACATATGATTTTAATTTATAAATTTGCATCGTTAATTATGATCAATCTTTTTTTA
TTTACTTATTTATATTTATTTTGTGTTGAAATTTTTAGGAGTGGCTCTGGGGGCAAAGAA
TTGGGTGAGGATATCGATA

Last Updated on 06.12.2018 16:11