Sequences from confirmation amplicon


Confirmation sequences for line 284G05 and hit At3g52360 are shown.
Line ID 284G05
Locus At3g52360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19412479
Primers used for PCR 20gp - 8474, show primer details
   
Sequence Name 18-K112307-0022-284-G05C-20gp
GenBank Accession LN494369 [GenBank]
Sequencing primer gene specific primer, 20gp: 5'-AAAATGTTGTTTGTTTGTTGACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K112307-0022-284-G05C-20gp
GATTTTTTTAAAAGGCAGTGTATATAAAAACCTTTGTCGTTCTCAAATCCTCTCTGTAAT
TCTGGTTTTTCTTGCAATGCTTCTTCCATTTCTCTCCTCCTCTCTCCTCCTCCGTCTCTC
TTCTCCGATCATGTCCCCTGATCCCAAATCCAATACCTTCTTCTTCTCTTTTTCCAGTTG
ATTACCGGAGAATTTCAGATCGCGATCTCCGATTTATTAATAATTACAGAAAAAAATAAT
TAAACCCTAGAGAGAGAGAGAGATATATATATACATGGAGAGTTCATTAGGTTTCATGGC
GGTTTTCGCCGTCTCAGGAAGCGTTGTGTTCTTAGCGAGTCAATTTCACAAGCGTCTTCT
CTCCGATTACATGGACAAGTTCGAATTCGAAATCCGTACGAACAAAATCCCAAAGGATAG
ATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCG
   
Sequence Name 02-K112306-0022-284-G05C-8409
GenBank Accession LN487099 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K112306-0022-284-G05C-8409
TCATTGAATATATCCTGAATCTATCCTTTGGGATTTTGTTCGTACGGATTTCGAATTCGA
ACTTGTCCATGTAATCGGAGAGAAGACGCTTGTGAAATTGACTCGCTAAGAACACAACGC
TTCCTGAGACGGCGAAAACCGCCATGAAACCTAATGAACTCTCCATGTATATATATATCT
CTCTCTCTCTCTAGGGTTTAATTATTTTTTTCTGTAATTATTAATAAATCGGAGATCGCG
ATCTGAAATTCTCCGGTAATCAACTGGAAAAAGAGAAGAAGAAGGTATTGGATTTGGGAT
CAGGGGACATGATCGGAGAAGAGAGACGGAGGAGGAGAGAGGAGGAGAGAAATGGAAGAA
GCATTGCAAGAAAAACCAGAATTACAGAGAGGATTTGAGAACGACAAAGGTTTTTATATA
CACTGCCTTTTTTAAAAAAATCAATTTTAAGTAATTTTGCTTTTCTTATTTGGTCAACAA
A

Last Updated on 06.12.2018 16:11