Sequences from confirmation amplicon


Confirmation sequences for line 285D01 and hit At1g76120 are shown.
Line ID 285D01
Locus At1g76120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28560197
Primers used for PCR 11dl - 8474, show primer details
   
Sequence Name 27-K109936-0022-285-D01C-11dl
GenBank Accession LN498433 [GenBank]
Sequencing primer gene specific primer, 11dl: 5'-CATTTGGGCTTGAGACTAATAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K109936-0022-285-D01C-11dl
GCCAGTTAAGAGCGGTCTGATATCAAAAACGCCGTCGTAGAGAAGCATACCTCAGTCGTC
TTCAAGGGTTTCGACTTTTGTGCGGTAGCCATTAACGTTGTTGCAGAGGAAAGCTGGAAA
ATAGAGAGTTCGATGAACTCTTGTTACTATAATGGAGGCTCAGGAGTTTGAAAACCGAGA
GATTTCCCCCGGTTCGAGGTTCCTGATTGAGAATCAGATCAGAAGTAGCTCAATATGTTG
ACAACTACCACCGATGAGGAAATAGACTTGTCCTGCGACGGAGGTGTCACGGAGCCTCAA
AAAGTTGCTATAATCTTCGCGTTTTGTGGTGTCCCGGCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 11-K109935-0022-285-D01C-8409
GenBank Accession LN491161 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K109935-0022-285-D01C-8409
TCGGACATGACCATTTACAATTGAATATATCCTGCCGGGACACCACAAAACGCGAAGATT
ATAGCAACTTTTTGAGGCTCCGTGACACCTCCGTCGCAGGACAAGTCTATTTCCTCATCG
GTGGTAGTTGTCAACATATTGAGCTACTTCTGATCTGATTCTCAATCAGGAACCTCGAAC
CGGGGGAAATCTCTCGGTTTTCAAACTCCTGAGCCTCCATTATAGTAACAAGAGTTCATC
GAACTCTCTATTTTCCAGCTTTCCTCTGCAACAACGTTAATGGCTACCGCACAAAAGTCG
AAACCCTTGAAGACGACTGAGGTATGCTTCTCTACGACGGCGTTTTTGATATCAGACCGC
TCTTTAACTGGGCCTATAATTTTGTTTTATATATGGGCTTATTAGTCTCAAGCCCAAATG

Last Updated on 06.12.2018 16:11