Sequences from confirmation amplicon


Confirmation sequences for line 285D09 and hit At5g40930 are shown.
Line ID 285D09
Locus At5g40930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16405587
Primers used for PCR DL73 - 8474, show primer details
   
Sequence Name 01-K018603-022-285-D09C-DL73
GenBank Accession LN486382 [GenBank]
Sequencing primer gene specific primer, DL73: 5'-TGTATGTAGTATTGGAGCCTGCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K018603-022-285-D09C-DL73
CATAATCCTTGCTTCACTATATACTGTGTTTTTCTAGACTAAAAACTTCATCTATAGGCA
GAACATGATTCAGATGCACTATTCTCCTTGTATGAGTAGATAAATTCGAACCACCTAGAA
GTTACTATCGCTAGAATAAGATGAAATGTTAATCTTCAAAGCTAGAACCTGATCATGGAC
AAATATAGTACAAGGAAAAGAAAACTGATATTTTTGTTAACCAAATGATGGTACGGTACC
CAACGAGAAACAGGTTACATAGGGAGTCTTTGTCAGAGCTAAGCGATGGGGGAGAAGAAG
AAGGTGATTATGAAGAGTACTCTACTGAGTTAGGCATAATGTCTTCACTAATTTGTATGT
TAATTTCCAAAGGACAATATGAGTTCTTATACATTTTCTGAGTCTCTCTCATTCTTAACT
TCAAATATGTTCTAGGAGTTGTTATAATGAGAAAACTCTTTTGTTTCAAAATGCATTAAA
AAAAAAGTGCTGGTCTTTAGACGCTCTTGGTGAGCGTTACTGCCTTGACACCGGCGTCTG
AGAATTGGCAAAACTGATCCACGCAACAACGTAACTGGCTAAGATGACCCATCCGAACAC
ATCATACTTGAACTCACTGGTCTTTTTCTGCTTCATCGTCTGTGAGAAAGAACAAACGTG
TTAAATTTTCATCAAACCAAATTGTAGTTTCGAATAAAAGAAACAACCTTCGCACTGTTA
GATGATGGTCCAGC
   
Sequence Name 02-K018603-022-285-D09C-8409
GenBank Accession LN486835 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K018603-022-285-D09C-8409
TTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAACTACATACAGGCGGCACCG
CTGGACCATCATCTAACAGTGCGAAGGTTGTTTCTTTTATTCGAAACTACAATTTGGTTT
GATGAAAATTTAACACGTTTGTTCTTTCTCACAGACGATGAAGCAGAAAAAGACCAGTGA
GTTCAAGTATGATGTGTTCGGATGGGTCATCTTAGCCAGTTACGTTGTTGCGTGGATCAG
TTTTGCCAATTCTCAGACGCCGGTGTCAAGGCAGTAACGCTCACCAAGAGCGTCTAAAGA
CCAGCACTTTTTTTTTAATGCATTTTGAAACAAAAGAGTTTTCTCATTATAACAACTCCT
AGAACATATTTGAAGTTAAGAATGAGAGAGACTCAGAAAATGTATAAGAACTCATATTGT
CCTTTGGAAATTAACATACAAATTAGTGAAGACATTATGCCTAACTCAGTAGAGTACTCT
TCATAATCACCTTCTTCTTCTCCCCCATCGCTTAGCTCTGAAAAGACTCCCTATGTAACC
TGTTTCTCGTTGGGTACCGTACCATCATTTGGTTAACAAAAATATCAGTTTTCTTTTCCT
TGTACTATATTTGTCCATGATCAGGTTCTAGCTTTGAAGATTAACATTTCATCTTATTCT
AGCGATAGTAACTTCTAGGTGGTTCGAATTTATCTACTCATACAAGGAGAATAGTGCATC
TGAATCATGTTCTGCCTATAGATGAAGTTTTTAGTCTAGAAAAACACAGTATATAGTGAA
GCAATGGTTATGACTTATG

Last Updated on 06.12.2018 16:11