Sequences from confirmation amplicon


Confirmation sequences for line 286A06 and hit T22O13 are shown.
Line ID 286A06
Locus T22O13 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11617875
Primers used for PCR 19qi - 8474, show primer details
   
Sequence Name 21-K115435-0022-286-A06C-19qi
GenBank Accession LN495790 [GenBank]
Sequencing primer gene specific primer, 19qi: 5'-CTGTGTATTTCAGGATTGGGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K115435-0022-286-A06C-19qi
GTTAAGTCTGGTTGCTTTCACTTCTTGAAGTATATAAGAAACGAAACGTTCAATCTTCTC
TTTCTTTCATTTGTATGAAAGATCTATTCTAATATAACAAATATCGAAGAATCGAATGGG
AAATGAAGTCTTTTGTTGGACAAGGTGAACTTGTATTAGTATGGAAATCCGCTCCGCAGC
AACATTATCACTAGCTCATGTCGTCAGGCTCATTCTCCATTTTATCACAATACATATAGA
AGATTCAGTCAATCGGTTACCAAAATTAAGAAGTTAATTATTGTTCTTTATTAAGAAACG
AAAGTACTGGTTTTTCAGGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 05-K115434-0022-286-A06C-8409
GenBank Accession LN488478 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K115434-0022-286-A06C-8409
AAAAACCAGTACTTTCGTTTTCTTAATAAAGAACAATAATTAACTTCTTAATTTTGGTAA
CCGATTGACTGAATCTTCTATATGTATTGTGATAAAATGGAGAATGAGCCTGACGACATG
AGCTAGTGATAATGTTGCTGCGNGAGCGGATTTCCATACTAATACAAGTTCACCTTGTCC
AACAAAGACTTCATTTCCCATTCGATCTTCGATATTTGTTATATTAGAATAGATCTTTCA
TACAAATGAAAGAAAGAGAGATTGAACGTTTCGTTTCTTATATACTTCAAGAGTGAAGCA
ACCAACTTAACTTAATTATAGAAACCTTTGTTCTGACATTTTACCTCATCCGTTAAAATA
AATAAATATGGGCCTGATCGGAAAAAGACCCAATC

Last Updated on 06.12.2018 16:11