Sequences from confirmation amplicon


Confirmation sequences for line 288A08 and hit At1g59660 are shown.
Line ID 288A08
Locus At1g59660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21928628
Primers used for PCR CQ34 - 8474, show primer details
   
Sequence Name 11-K105632-0022-288-A08C-CQ34
GenBank Accession LN491111 [GenBank]
Sequencing primer gene specific primer, CQ34: 5'-TTTTCTGAATTTTGGCTTTGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K105632-0022-288-A08C-CQ34
TCTTTCACCTGTTTGTTTCACCAATGTTTGTCCAGGTTCCATTCTTCAGCGATGAAGAGG
AGAATTCCAGTACACCAAAGGCTGATGCGTTTTTCATTCCAAGGGAGAACCCTAGAGCTT
TATTTATACGCCCAGTTGAAAGGGTAAAATCAGAACATCCAAAGGATAGCCCGACCCCAC
TGCAGGAGAACGGTAAGCTTCCCTTTTTTTTTTCATTCATTGGCTTATTTATTGCAAGAT
TTAAAGCTTTGTTTCAATTGCAAAAAAAAGCTTTTCATTGTCCCAATAGCTCGAAAAATG
ATCTGCTTGCATATTAATATAGTGGCTGAATTTGATCATATCTAACCCCTTATTACATTT
GAAATTCAGGTAAAAGGTCAAATGGGGTTACTAATGGACCCAACCATGAGAGTGAGTATT
TTAAATATGCATTTGTAACAAAATTGTGATTAAAGTTTGTGCATTGGTGAGGCCATTCAC
TGACTCTACTGTTAATATGTGCAGCTAAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCACCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCATTTTTTTTTCATTTCAAAAATGTAAATTTCC
   
Sequence Name 27-K105633-0022-288-A08C-8409
GenBank Accession LN498385 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K105633-0022-288-A08C-8409
CCATTTACATTGAATATATCCTTAGCTGCACATATTAACAGTAGAGTCAGTGAATGGCCT
CACCAATGCACAAACTTTAATCACAATTTTGTTACAAATGCATATTTAAAATACTCACTC
TCATGGTTGGCTCCATTAGTAACCCCATTCGACCTCTTACCTGAATTTCAAATGTAATAA
GGGGTCAGATATGATCAAATTCAGCCACTATATTAATATGCAAGCAGATCATCTTCCGAG
CTATTGGGACAATGAAAAGCTTCTTTTTGCAATTGAAACAAAGCTCTAAATCTTGCAATA
AATAAGCCAATGAATGAAAAAAAAAAGGGAAGCTTACCGTTCTCCTGCAGTGGGGTCGGG
CTATCCTTTGGATGTTCTGATTTTACCCTTTCAACTGGGCGTATAAATAAAGCTCTAGGG
TTCTCCCTTGGAATGAAAAACGCATCACCCTTTGGTGTACTGGAATTCTCCTCTTCATCG
CTAAAAAATGGAACCTGGACAAACATTGGTGAAACAAACAGGTAGAAAGACCTTCAGTAA
ACATCACTAAAGACTGAAATATTCAAAACAAAGCCA
Primers used for PCR 20ah - 8474, show primer details
   
Sequence Name 40-K118837-0022-288-A08C-20ah
GenBank Accession LN503880 [GenBank]
Sequencing primer gene specific primer, 20ah: 5'-AGTCTACACACATCCGTCTCTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K118837-0022-288-A08C-20ah
ACAACAAGTCCTTTACCCGCTTGCAATAACCCTGTTCAACCCTTTCTTTTGCAGCCAGTT
CTTGGATCCGAGGCTCTGTGAAATATTCAGAGTGATGCAACTTCGGCATTAATGACTCAA
TATCTGCTCCACTTGGTGAACTGTGTGAACCGTTTTTGTCGCCTCCGTGATTTTCATGTG
TCCCGTTCTGTTTCTGATTGACTTTTACCGGGGGAGCTTCACGAATTAACAGTAAATATG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATT
   
Sequence Name 16-K118836-0022-288-A08C-8409
GenBank Accession LN493642 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K118836-0022-288-A08C-8409
ATTTACATTGAATATTCCATATTTACTGTTAATTCGTGAAGCTCCCCCGGTAAAAGTCAA
TCAGAAACAGAACGGGACACATGAAAATCACGGAGGCGACAAAAACGGTTCACACAGTTC
ACCAAGTGGAGCAGATATTGAGTCATTAATGCCGAAGTTGCATCACTCTGAATATTTCAC
AGAGCCTCGGATCCAAGAACTGGCTGCAAAAGAAAGGGTTGAACAGGGTTATTGCAAGCG
GGTAAAGGACTTTGTTGTTGGGAGACACGGTTACGGGAGCATAAAGTTTCTAGGAGAGAC
GGATGTGTGTAGACT

Last Updated on 06.12.2018 16:11