Sequences from confirmation amplicon


Confirmation sequences for line 289C02 and hit At1g09420 are shown.
Line ID 289C02
Locus At1g09420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3039364
Primers used for PCR KS21 - 8474, show primer details
   
Sequence Name 36-K032520-022-289-C02C-8409
GenBank Accession LN502113 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K032520-022-289-C02C-8409
GTGTGATCTTGACGGTGTAGACCCTACATATTGTGCAGCAGCCTTGTATATTGATAATGC
ACGTTGGGATGGTGTACCTTTCCTTGTAAGAGTTGGCACTGGCCTCATTAAACACAGGTA
AGAATAATTATTCCTACTCATAGACATATCCAACTGTCATAACAATTGATTGGTGTGCCT
TAATGTTGAAAGAGTGGAGATTCATGTGCAATTCCGGCATGTTCCTGGAAACCTATACCG
AGAAAATATTGGAATAAACATAGATTTGGGGACAAATGAGCTTATTCTACGTGACGAACC
TGATGAGGCCATCCTGGTGAAAATCAACAACAAGGTTCCTGGTTTAGGTCTTCAGCTAGA
CGCTTCTGAACTTAACTTGCTCTATAAAGACAGGCAAGTTTTGCATTCTCATCTTTGTTC
CTACACATTTTAAAAATCGAACTTTTGCGCCTTATGGTTGGTTGAATTGTTGGATCAAAC
CTGTAGGTATAAAACCGAAGTACCAGACTCATATGAACACCTAATTCATGATGTAATTGA
CGGAGACAATCATCT
   
Sequence Name 36-K032520-022-289-C02C-KS21
GenBank Accession LN502114 [GenBank]
Sequencing primer gene specific primer, KS21: 5'-TCTCATGAACAGATGATTGTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K032520-022-289-C02C-KS21
ATGAGTCTGGGTACTTCGGTTTTATACCTACAGGGTTTGATCCAACAATTCAACCAACCA
TAAGGCGCAAAAGTTCGATTTTTAAAATGTGTAGGAACAAAGATGAGAATGCAAAACTTG
CCTGTCTTTATAGAGCAAGTTAAGTTCAGAAGCGTCTAGCTGAAGACCTAAACCAGGAAC
CTTGTTGTTGATTTTCACCAGGATGGCCTCATCAGGTTCGTCACGTAGAATAAGCTCATT
TGTCCCCAAATCTATGTTTATTCCAATATTTTCTCGGTATAGGTTTCCAGGAACATGCCG
GAATTGCACATGAATCTCCACTCTTTCAACATTAAGGCACACCAATCAATTGTTATGACA
GTTGGATATGTCTATGAGTAGGAATAATTATTCTTACCTGTGTTTAATGAGGCCAGTGCC
AACTCTTACAAGGAAAGGTACACCATCCCAACGTGCATTATCAATATACAAGGCTGCTGC
ACAATATGTAGGGTCTACACCGTTCAAGATCACACCGTTCTTGTCTCGGGAACTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAACTTTATTTCGACGTG

Last Updated on 06.12.2018 16:11