Sequences from confirmation amplicon


Confirmation sequences for line 289C07 and hit At2g41850 are shown.
Line ID 289C07
Locus At2g41850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17463557
Primers used for PCR KS84 - 8474, show primer details
   
Sequence Name 36-K101501-0022-289-C07C-8409
GenBank Accession LN502160 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K101501-0022-289-C07C-8409
CTTCGTATTAAATGGCTTCATCGTATTTACAATTTCTTGTAATTACCTGAACGGTGAGAA
TAGAATTGCATGGACCAGTTAATTGAATAGACTTAAGGAGATAAGTATTCCCTTTAGGAA
CTAGGAGATTAACAGCTCCATTTGAAGAACATGCTTTCTTCCACGCATTCACGAACGCCT
AAACAATGCATGCATGGATCATTAGTACTAAAAACAGTTTTCTAATTAATTATACGATGA
AATAAAATAAAAACATTTTTATGTGTATATATACACCTGCGTGTCATCGGTTTTTCCATC
TCCTTTAGCTCCAAAATCAGAAACACTAACGGTGGTTGGAGTCCGCAAAGAGGCTCTTAC
AAAACTTCTCAATCCC
   
Sequence Name 36-K101505-0022-289-C07C-KS84
GenBank Accession LN502161 [GenBank]
Sequencing primer gene specific primer, KS84: 5'-CGGGATTGAGAAGTTTTGTAAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K101505-0022-289-C07C-KS84
TTTCTGATTTTGGAGCTAAGGAGATGGAAAAACCGATGACACGCAGGTGTATATATACAC
ATAAAAATGTTTTTATTTTATTTCATCGTATAATTAATTAGAAAACTGTTTTTAGTACTA
ATGATCCATGCATGCATTGTTTAGGCGTTCGTGAATGCGTGGAAGAAAGCATGTTCTTCA
AATGGAGCTGTTAATCTCCTAGTTCCTAAAGGGAATACTTATCTCCTTAAGTCTATTCAA
TTAACTGGTCCATGCAATTCTATTCTCACCGTTCAGGTAATTACAAGAAATTGTAAATAC
GATGAAGCCATTTATATACGAAGCCATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CANAAATGTAGATTTCCG

Last Updated on 06.12.2018 16:11