Sequences from confirmation amplicon


Confirmation sequences for line 289D11 and hit At1g55810 are shown.
Line ID 289D11
Locus At1g55810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20863844
Primers used for PCR RZ16 - 8474, show primer details
   
Sequence Name 07-K018603-022-289-D11C-RZ16
GenBank Accession LN489050 [GenBank]
Sequencing primer gene specific primer, RZ16: 5'-CTCATGGTTCTTGTTAAGGGAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K018603-022-289-D11C-RZ16
AGAAGCGGGAGGAGAGAGAAATTGAGAGAGCAATTGAAATTGGAAGCGCGAGTCCCTCCG
TATTTATTTATTTACTTACCAAGATAATAATAATGTACCGCTTCACTGTCGGTAACTTTT
ATACGCTCACGTCACTTCATTGCACACCAAAAGACTCTTTAAAGTTTTACATTTTGTATG
ACTTCAACAGTCACCCAATTATAGAGAGACACGTGGACGATGACCCAAAAACTGTGAATA
CGTTCACACTCAGTTTGTTTTGATATTACCGTATACATATGTGTGAATCCGATCATAAAA
GTACTACGTATAACAGTTGAAACAAAAGAAATCAAAATTTTCGAGGGAGGCAAGGCAAGC
TCGCACTCTCTCACTCATCATCGGTTCCAAAATAGCGGTCTCCAAACTCGCCCATACCAG
GAACAACTCGGAACTCGTCGTTTAAACCGAGTTCTATCTCTGAAGTCACAATCTTTATCC
TTGGGAACTTTTTGCACACCACATTTACTCCTTCAGGTGCCTATACCATATTAAATCTAC
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTA
   
Sequence Name 08-K018603-022-289-D11C-8409
GenBank Accession LN489518 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K018603-022-289-D11C-8409
TAGATTTCCCGGACATGAAGCCATGTAGATTTAATATGGTATAGGCACCTGAAGGAGTAA
ATGTGGTGTGCAAAAAGTTCCCAAGGATAAAGATTGTGACTTCAGAGATAGAACTCGGTT
TAAACGACGAGTTCCGAGTTGTTCCTGGTATGGGCGAGTTTGGAGACCGCTATTTTGGAA
CCGATGATGAGTGAGAGAGTGCGAGCTTGCCTTGCCTCCCTCGAAAATTTTGATTTCTTT
TGTTTCAACTGTTATACGTAGTACTTTTATGATCGGATTCACACATATGTATACGGTAAT
ATCAAAACAAACTGAGTGTGAACGTATTCACAGTTTTTGGGTCATCGTCCACGTGTCTCT
CTATAATTGGGTGACTGTTGAAGTCATACAAAATGTAAAACTTTAAAGAGTCTTTTGGTG
TGCAATGAAGTGACGTGAGCGTATAAAAGTTACCGACAGTGAAGCGGTACATTATTATTA
TCTTGGTAAGTAAATAAATAAATACGGAGGGACTCGCGCTTCCAATTTCAATTGCTCTCT
CAATTTCTCTCTCCTCCCGCTTCTGCTTCTCTCCCTTAA

Last Updated on 06.12.2018 16:11