Sequences from confirmation amplicon


Confirmation sequences for line 290G04 and hit At1g17720 are shown.
Line ID 290G04
Locus At1g17720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6094875
Primers used for PCR CQ40 - 8474, show primer details
   
Sequence Name 06-K105950-0022-290-G04C-CQ40
GenBank Accession LN488816 [GenBank]
Sequencing primer gene specific primer, CQ40: 5'-GCAGCTTCAGTACTTCCTTGAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K105950-0022-290-G04C-CQ40
TGCTGGTCACCGGAGATAAGTATAATAAATTGGTCATTAGTTTATTCCTTATACAAGACA
GAAAAGTACAGCAATGACCAGAAGACAAGCAAGTCAACCTGTAAGAACCTGTTGCTACCC
TCAATCCATCGCCACTCAGACAACACTCAAATTTATCAAAGATGGAGTCATTTTCATATA
GATCGCATAGCTGCAACCGGGTAAAACATAACGTGGTTAGCTTTCAACACAGAATCAACA
AATAAAAGATGCCACAGAAAGAAGCTCTCACCCTGGGTCTCAGATGTTCATGAACCTGGT
AAGATGCGACCGGACCAGAATCCATGTTTATGTCCCACAGCTGAAACGTCAAAAAGGGTA
AAAGATATCAGAAAGAACTAAAGAATAGACCTCTATTGGCCCACAATCAGAAAAATATAC
ACAAAGGGAACCGAATGATTTCCAACCTTGAGCGTCATGTCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 22-K105951-0022-290-G04C-8409
GenBank Accession LN496116 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K105951-0022-290-G04C-8409
GGAATGAGCCATTTACATTGAATATATCCTGACATGACGCTCAAGGTTGGAAATCATTCG
GTTCCCTTTGTGTATATTTTTCTGATTGTGGGCCAATAGAGGTCTATTCTTTAGTTCTTT
CTGATATCTTTTACCCTTTTTGACGTTTCAGCTGTGGGACATAAACATGGATTCTGGTCC
GGTCGCATCTTACCAGGTTCATGAACATCTGAGACCCAGGGTGAGAGCTTCTTTCTGTGG
CATCTTTTATTTGTTGATTCTGTGTTGAAAGCTAACCACGTTATGTTTTACCCGGTTGCA
GCTATGCGATCTATATGAAAATGACTCCATCTTTGATAAATTTGAGTGTTGTCTGAGTGG
CGATGGATTGAGGGTAGCAACAGGTTCTTACAGGTTGACTTGCTTGTCTTCTGGTCATTG
CTGTACTTTTCTGTCTTGTATAAGGAATAAACTAATGACCAATTTATTATACTTAATCTC
CGGTGAACCAGCAATCTATTCCGCGTATTTGGAGCGTCTCAAGGAAT

Last Updated on 06.12.2018 16:11